High-resolution capillary gel electrophoresis of the B

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High-resolution capillary gel electrophoresis of the B High-resolution capillary gel electrophoresis of the B. suis-targeted quantitative real-time PCR amplicons. High-resolution capillary gel electrophoresis of the B. suis-targeted quantitative real-time PCR amplicons. Primer B. suis for and B. suis rev were used for amplification of the targeted region within BS1330_II0657 locus. Lack of the 17 bp repeat in Brucella spp. or B. suis bv 5 are visualised and resulted in a fragment size of 106 bp for B. suis bv 1–4 and 89 bp for all other strains. Lane C2: Size marker; lane B1: B. suis bv 1; lane B2: B. suis bv 2; lane B3: B. suis bv 3; lane B4: B. suis bv 4; lane A5: B. suis bv 5; lane B10: B. abortus 544; lane A10: B. melitensis 16M; lane B9: B. ovis; lane B11: B. canis; lane A9: B. microti; lane A11: no template control C. Hänsel et al. Vet Rec Open 2015;2:e000084 ©2015 by British Veterinary Association