Effects of levonorgestrel, medroxyprogesterone acetate, norethindrone, and 17β- estradiol on vascular endothelial growth factor isomers 121 and 165 in.

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Presentation transcript:

Effects of levonorgestrel, medroxyprogesterone acetate, norethindrone, and 17β- estradiol on vascular endothelial growth factor isomers 121 and 165 in Ishikawa cells David F Archer, M.D., Francisco J Navarro, M.D., Susan Leslie, B.S., Sebastian Mirkin, M.D. Fertility and Sterility Volume 81, Issue 1, Pages 165-170 (January 2004) DOI: 10.1016/j.fertnstert.2003.05.028

Figure 1 Representative ethidium-bromide–stained polyacrylamide gel of reverse-transcribed ER and PR mRNA coamplified by PCR from Ishikawa cells (Ish), human endometrium (Endo), and human ovary (Ov). The expected products from the ER and PR migrate at 380 and 535 bp, respectively. Archer. Progestin's effect on VEGF in Ishikawa cells. Fertil Steril 2004. Fertility and Sterility 2004 81, 165-170DOI: (10.1016/j.fertnstert.2003.05.028)

Figure 2 Effect of levonorgestrel, MPA, norethindrone, and 17β-E2 on VEGF 165 isoform abundance using cpm ratios vs. β-actin. Relative abundance of VEGF 165 mRNA from control and three different concentrations of levonorgestrel (LNG), MPA, norethindrone (NET), and 17β-E2 (E2) are indicated. Results are expressed as means ± SE. *P<.05 as compared with the control group. ±P<.05 as MPA at 1.0 μM is compared with E2 at 1.0 μM. **P<.05 as norethindrone at 1.0 μM is compared with E2 at 1.0 μM. Archer. Progestin's effect on VEGF in Ishikawa cells. Fertil Steril 2004. Fertility and Sterility 2004 81, 165-170DOI: (10.1016/j.fertnstert.2003.05.028)

Figure 3 Effect of levonorgestrel, MPA, norethindrone, and 17β-E2 on VEGF 121 isoform abundance using cpm ratios vs. β-actin. Relative abundance of VEGF 121 mRNA from control and three different concentrations of levonorgestrel (LNG), MPA (MPA), norethindrone (NET), and 17β-E2 (E2) are indicated. Results are expressed as means ± SE. *P<.05 as compared with the control group. ±P<.05 as MPA at 1.0 μM is compared with E2 at 1.0 μM. **P<.05 as norethindrone at 1.0 μM is compared with E2 at 1.0 μM. Archer. Progestin's effect on VEGF in Ishikawa cells. Fertil Steril 2004. Fertility and Sterility 2004 81, 165-170DOI: (10.1016/j.fertnstert.2003.05.028)