Analysis of Cdc7p at the end of meiosis.

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Analysis of Cdc7p at the end of meiosis. Analysis of Cdc7p at the end of meiosis. (A) The indicated strain was imaged during late meiosis. The two frames were taken 5 minutes apart. While the FSM is incomplete (left panel, left cell), Cdc7p–CHY (red) is present on the SPBs, and it is absent after the leading-edge complex has dissociated, indicating closure of the FSM. (B) Tetranucleated cells of the indicated strains were imaged. Cdc7p–GFP is absent from the SPBs in these cells. Scale bars: 10 µm. (C,D) The indicated mutants, expressing Cdc7p–GFP in a pat1-114 background, were induced to undergo meiosis by incubation at 32°C. Western blots were probed with an antibody to detect the GFP tag. Graphs and western blots are labelled as described for Fig. 3. Andrea Krapp, and Viesturs Simanis J Cell Sci 2014;127:3149-3161 © 2014. Published by The Company of Biologists Ltd