Effects of DWJM on UCB CD34+-derived cell subsets.

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Effects of DWJM on UCB CD34+-derived cell subsets. Effects of DWJM on UCB CD34+-derived cell subsets. In these experiments, enriched UCB CD34+ cells 3 × 104 were seeded onto DWJM or cultured in suspension for 7 days. Cells adherent to DWJM were isolated using collagenase treatment as described previously. The DWJM adherent and nonadherent populations were analyzed separately by flow cytometry. The total number of cells after culture was measured using automated cell counter. The absolute number of a specific population was measured by multiplying the total cell number of the specific condition with the percentage of the specific population in the parent population as determined by flow. Data were log2 transformed. (A) Percentage of live CD34+ cells on day 7 in suspension, DWJM-adherent, and nonadherent populations. (B) Percentage of live CD34+CD38− cells on day 7 in suspension, DWJM-adherent, and nonadherent populations. (C) Percentage of live MMP cells on day 7 in suspension, DWJM-adherent, and nonadherent populations. (D) Percentage of live HSCs on day 7 in suspension, DWJM-adherent, and nonadherent populations. (E) Fold change in CD34+ cells in suspension vs DWJM culture conditions. (F) Fold change in CD34+CD38− cells in suspension vs DWJM culture conditions. (G) Fold change in MPP cells in suspension vs DWJM culture conditions. (H) Fold change in HSCs in suspension vs DWJM culture conditions. *P < .05, **P < .01, and ***P < .001. Dandan Li et al. Blood Adv 2019;3:1011-1026 © 2019 by The American Society of Hematology