Quantitation by image analysis of global DNA methylation in human spermatozoa and its prognostic value in in vitro fertilization: a preliminary study

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Quantitation by image analysis of global DNA methylation in human spermatozoa and its prognostic value in in vitro fertilization: a preliminary study Mehdi Benchaib, M.D., Ph.D., Mounir Ajina, M.D., Jacqueline Lornage, M.D., Ph.D., Alain Niveleau, Ph.D., Philippe Durand, Ph.D., Jean François Guérin, M.D., Ph.D. Fertility and Sterility Volume 80, Issue 4, Pages 947-953 (October 2003) DOI: 10.1016/S0015-0282(03)01151-8

FIGURE 1 Evolution of DNA methylation according to the time of HCl exposure. For 15 and 30 minutes, the measurements are stable; from 45 minutes depurinization of DNA appeared. Benchaib. Sperm DNA methylation and IVF outcome.Fertil Steril 2003. Fertility and Sterility 2003 80, 947-953DOI: (10.1016/S0015-0282(03)01151-8)

FIGURE 2 Difference in DNA heterogeneity of sperm samples according to the fertilization rate. Benchaib. Sperm DNA methylation and IVF outcome.Fertil Steril 2003. Fertility and Sterility 2003 80, 947-953DOI: (10.1016/S0015-0282(03)01151-8)

FIGURE 3 Dispersion of DNA heterogeneity according to a cutoff of 30% compared with the fertilization rate (■ >30%, □ <30%). Benchaib. Sperm DNA methylation and IVF outcome.Fertil Steril 2003. Fertility and Sterility 2003 80, 947-953DOI: (10.1016/S0015-0282(03)01151-8)

FIGURE 4 DNA level (median) according to the IVF results. Benchaib. Sperm DNA methylation and IVF outcome.Fertil Steril 2003. Fertility and Sterility 2003 80, 947-953DOI: (10.1016/S0015-0282(03)01151-8)