Fernando M Reis, M. D. , Ph. D. , Ana Luiza Maia, M. D. , Ph. D

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Fig. 3. Representative micrographs of progesterone receptor-B immunostaining in the eutopic endometrium of the controls and patients with endometriosis.

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Presentation transcript:

Progestin modulation of c-fos and prolactin gene expression in the human endometrium Fernando M Reis, M.D., Ph.D., Ana Luiza Maia, M.D., Ph.D., Maria Flávia M Ribeiro, M.D., Ph.D., Poli Mara Spritzer, M.D., Ph.D. Fertility and Sterility Volume 71, Issue 6, Pages 1125-1132 (June 1999) DOI: 10.1016/S0015-0282(99)00138-7

FIGURE 1 Immunostaining of c-fos and PRL in human endometrium (A) c-fos proliferative/placebo. (B) c-fos secretory/placebo. (C) c-fos secretory/MPA. (D) PRL proliferative/placebo. (E) PRL secretory/placebo. (F) PRL secretory/MPA. Specific immunostaining is indicated by the brown color in the nuclei (A) and citosol (E, F). Original magnification: ×400. Fertility and Sterility 1999 71, 1125-1132DOI: (10.1016/S0015-0282(99)00138-7)

FIGURE 2 Percentage of patients with endometrial samples that showed positive immunostaining for c-fos (A) or PRL (B) on epithelial and stromal cells. ∗P<.05 vs. proliferative/placebo (Fisher’s exact test). MPA = medroxyprogesterone acetate; PRL = prolactin. Fertility and Sterility 1999 71, 1125-1132DOI: (10.1016/S0015-0282(99)00138-7)

FIGURE 3 Percentage of patients whose endometrial samples showed positive immunostaining for c-fos (A) or PRL (B) on epithelial (open bars) and stromal (hatched bars) cells according to endometrial biopsy dating. EP = early proliferative; MP = midproliferative; ES = early secretory; MS = midsecretory; LS = late secretory. Serum E2 levels and progesterone/E2 ratios of patients from each endometrial phase are expressed as medians (C). • = progesterone/E2; ○ = E2. Fertility and Sterility 1999 71, 1125-1132DOI: (10.1016/S0015-0282(99)00138-7)

FIGURE 4 Reverse transcription–polymerase chain reaction product visualized in agarose gel stained with ethidium bromide. Lanes 1–7 = proliferative/placebo; lanes 8–13 = secretory/placebo; lanes 14–18 = secretory/MPA. N = negative control. Quantitative results are expressed in relation to β2-microglobulin optical density (arbitrary units) and plotted as medians plus 75th percentiles. ∗P<.05 vs. proliferative/placebo (Dunn’s test). MPA = medroxyprogesterone acetate; PRL = prolactin; bp = base pair. Fertility and Sterility 1999 71, 1125-1132DOI: (10.1016/S0015-0282(99)00138-7)

FIGURE 5 Correlation between c-fos mRNA in the endometrium and serum E2 levels (A) and progesterone/E2 ratios (B). r = Spearman rank correlation coefficient). MPA = medroxyprogesterone acetate; mRNA = messenger RNA. Fertility and Sterility 1999 71, 1125-1132DOI: (10.1016/S0015-0282(99)00138-7)