CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. CD169+ macrophages mediate the transport of bacteria.

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CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. (A) Confocal images of spleen sections from WT or CD169-DTR mice at 6 hpi. Colocalization of CD11c and CD8α is shown in white. Lm are rendered with spots in green. White arrowheads indicate Lm in the MZ. Scale bars, 70 to 80 μm. (B) Confocal images of spleen sections from WT or CD169-DTR mice at 9 hpi. Spleen sections stained for Lm (green), CD169 (blue), and CD11c (red). Colocalization of CD11c and CD8α is shown in white. Lm are rendered with spots. White arrows indicate areas of Lm. Scale bars, 30 μm. (C) High-magnification confocal image showing Lm-infected CD169+ macrophages and CD8α+ DCs in close contact at 3 hpi. Spleen sections stained for Lm (green), CD169 (blue), CD11c (red), and CD8α (light blue). Scale bars, 20 μm. (D and E) Quantification of viable Lm recovered from FACS-sorted myeloid cells from pooled WT or CD169-DTR mice at (D) 1 hpi and (E) 3 hpi (n = 2 to 4). (F) General gating strategy used for CD169+ macrophages and DC subset identification from the spleen. (G) Representative confocal image of WT mice at 3 hpi. Spleen sections stained for Lm (white), CD169 (blue), and CD11c (red). Lm are rendered with spots. White and green arrowheads indicate Lm in CD169+ cells and CD169+CD11c+ cells, respectively. Scale bars, 20 μm. All data are representative of three to four separate experiments with two to four mice per group. Oriana A. Perez et al. Sci. Immunol. 2017;2:eaah5520 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works