FAM134B-2 induces starvation-induced ApoCIII degradation. FAM134B-2 induces starvation-induced ApoCIII degradation. (A–C) Quantification of colocalization of EGFP-ApoCIII and SERCA2 positive ER. FAM134B KO HeLa cells were infected with adenovirus containing mouse FAM134B-2 and transfected with pEGFP-ApoCIII. (A) Immunoblot analysis of FAM134B-2 in HeLa FAM134B KO cells. (B) Representative images of HeLa FAM134B KO cells. (C) Quantification of GFP-ApoCIII positive areas in SERCA2-positive ER. (D) Co-immunoprecipitation of endogenous GFP-ApoCIII, FLAG-FAM134B-2. FLAG-FAM134B-2 was transiently overexpressed in HEK293T cells. 24 h after transfection, the cells were starved for 6 h in the presence of EBSS and bafilomycin A1. (E, F) Quantification of GFP-FAM134B-2 and mCherry-ApoCIII double positive LAMP1. The cells were transfected with GFP-FAM134B-2 and mCherry-ApoCIII. 24 h after transfection, the cells were starved for 6 h in the presence of EBSS and bafilomycin A1 or cultured with complete media. (E) Representative images of HeLa cells. (F) Quantification of GFP-FAM134B-2 and mCherry-ApoCIII double positive LAMP1. (G, H) Quantification of mCherry-ApoCIII–positive LAMP1. FAM134B KO HeLa cells were transfected with GFP-FAM134B-2 and mCherry-ApoCIII. 24 h after transfection, the cells were starved for 6 h in the presence of EBSS and bafilomycin A1. (G) Representative images of HeLa cells. (H) Quantification of mCherry-ApoCIII–positive LAMP1. One-way ANOVA with a Student–Newman post hoc test was used for statistical analysis. *P < 0.05. Shohei Kohno et al. LSA 2019;2:e201900340 © 2019 Kohno et al.