Angiotensin II induces apoptosis in human mural granulosa-lutein cells, but not in cumulus cells Elisa Acosta, M.Sc., Óscar Peña, M.Sc., Frederick Naftolin,

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Presentation transcript:

Angiotensin II induces apoptosis in human mural granulosa-lutein cells, but not in cumulus cells Elisa Acosta, M.Sc., Óscar Peña, M.Sc., Frederick Naftolin, M.D, D.Phil., Julio Ávila, Ph.D., Angela Palumbo, M.D., Ph.D. Fertility and Sterility Volume 91, Issue 5, Pages 1984-1989 (May 2009) DOI: 10.1016/j.fertnstert.2008.04.026 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Fluorescence micrographs (×400). (A) Cumulus cells showing absence of apoptosis. (B) Mural granulosa-lutein cells after 24-h incubation without treatment. (C) Mural granulosa-lutein cells after 24-h incubation with angiotensin II 10−9 mol/L. Apoptotic cells are identified by green cytoplasm. Note the increased apoptosis caused by the addition of AngII. Fertility and Sterility 2009 91, 1984-1989DOI: (10.1016/j.fertnstert.2008.04.026) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Increased apoptosis of mural granulosa-lutein cells after 48-h culture in the presence of different doses of angiotensin II (solid columns) compared with untreated control cultures (clear columns). aMeans are statistically different (Student t test). Fertility and Sterility 2009 91, 1984-1989DOI: (10.1016/j.fertnstert.2008.04.026) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Blockade of the angiotensin II (AngII)–driven increase of granulosa cell apoptosis by simultaneous incubation with AngII and saralasin (a blocker of both AngII type 1 [AT1] and type 2 [AT2] receptors). (B) Blockade of the AngII-driven increase of granulosa cell apoptosis by simultaneous incubation with AngII and the specific AT2 receptor blocker CGP42112A. (C) Decrease of apoptosis by the AngII receptor blockers alone: Note that the specific AT2 blocker is more effective than saralasin. aMeans are statistically different (Student t test). Fertility and Sterility 2009 91, 1984-1989DOI: (10.1016/j.fertnstert.2008.04.026) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions