Fig. 2. Analysis of morphology, pluripotency marker expression and transgene silencing in the colonies emerging during reprogramming. Analysis of morphology,

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Immuno-gold localisation of COPI in N. gruberi cells.

Volume 4, Issue 6, Pages (June 2009)

Volume 4, Issue 2, Pages (February 2015)

Volume 3, Issue 3, Pages (September 2008)

Volume 4, Issue 1, Pages (January 2009)

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 4. Phosphorylation of the MARCKS-homology domain partially inhibits Hts' ability to disrupt Dlg postsynaptic targeting to the larval NMJ.(A–D″) High.

Fig. 1. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice.

Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle

TC-1 silencing sensitized A549 and SPC-A-1 cells to radiation therapy

Fig. 7. Knockdown of Meis1 abolishes CR4. 2-GFP expression

Fig. 4. BMP and nodal induce invasion of metastatic and radial growth phase melanoma cells in human epidermal skin reconstructs. BMP and nodal induce invasion.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Fig. 4. Increased adipocyte differentiation in Cbx7-KO ES cells

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Fig. 2. Transfection and clonal selection of rat pluripotent stem cells to generate stable transgenic lines. Transfection and clonal selection of rat pluripotent.

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. E-cadherin localizes in nano-scale clusters.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Differentiation of neural crest cells into corneal endothelial cells

Rescue of motoneuron degeneration by small molecules in ATXN3-CAG89 transgenics. Rescue ofmotoneurondegeneration by small molecules in ATXN3-CAG89 transgenics.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

Fig. 3. Low power hemi retinal image of the RPE surface showing the albino central and equatorial retina and pigment distribution in the periphery. Low.

Fig. 4. Non-autonomous rescue of puc expression in DME cells

Fig. 5. Morphological changes of the N

Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.

Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 2. Morphological analysis of acentriolar mitotic spindles

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 6. CBX7 expression in adipocyte differentiation of adipose-derived stem cells.(A) The adipose-derived stem cells, ADS1, were analyzed for the capability.

Co-development of epithelium and vasculature in early developing submandibular gland. Co-development of epithelium and vasculature in early developing.

Fig. 7. Lhx1-RNAi reduces the eye size

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 1. Aboveground biomass of Caragana and herbaceous plants, and proportional abundance of Caragana, under different grazing management treatments. Aboveground.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

The derivation of hESCs and hiPSCs.

Fig. 2. Sorting in chimeric embryoid bodies of wildtype with E- or N-cadherin null ES cells.Wildtype, E-cadherin null (9J), and N-cadherin null (Ncad95)

Fig. 6. Apical polarity of primitive endoderm cells on surface of embryoid bodies.Embryoid bodies were analyzed by immunofluorescence microscopy for GATA4.

Fig. 8. The morphology of the ventral nerve cord in Ror-Myc overexpressing embryos is normal. The morphology of the ventral nerve cord in Ror-Myc overexpressing.

Statistical chart of significantly differentially expressed genes

Fig. 4. Expression analysis of Onecut transcription factors during mdDA neuron development.Adjacent coronal sections of E11.5, E12.5 and E13.5 mouse brains.

Effect of Dipy on the expression of chondrogenic markers.

Histological analyses for limb-specific Uhrf1 KO mice.

Delay of tail resorption in trβ crispants during natural metamorphosis

Fig. 2. Effects of pH on percentage of motile spermatozoa and VAP after 1 and 5 min after activation. Effects of pH on percentage of motile spermatozoa.

Fig. 6. IIS affected the soma area of most neurons in pharate adults, but not in larvae.(A,B) Pan-peptidergic expression pattern of 386-Gal4,UAS-CD8::GFP.

Morphological changes induced by T3 treatment in trβ crispants

Fig. 3. transparent is required cell-autonomously in iridophores

Fig. 2. Expression of Cx43 mutant T154A resulted in non-radial spreading and formation of protrusions in J558µm3 cells spreading in response to BCR signaling.(A)

Fig. 4. CR4.2 may be active in amacrine cells but not in ganglion cells.Chick retinas were electroporated with either the control CAG-GFP construct or.

Altered intracellular distributions of acetylated α-tubulin, mitochondria and peroxisomes. Altered intracellular distributions of acetylated α-tubulin,

Fig. 1. Generation of induced pluripotent stem cells (iPSCs) from urine cells (UC). Generation of induced pluripotent stem cells (iPSCs) from urine cells.

Fig. 5. Upregulation and subcellular relocalization of Hsp70 in striated muscles overexpressing either DVAP-V260I or DVAP-WT constructs.(A) NMJs of BG57-Gal4/+

mip120 null egg chambers have a condensed nurse cell DNA phenotype

Depletion of COPI protein inhibits cis to trans cisternal maturation.

Fig. 2. Defective development of lymphatic vessels in zebrafish embryos treated with cigarette smoke extract or snuff extract. Defective development of.

Fig. 4. Representative still images of behaviour and characteristics typical of inshore foraging strategy of Australasian gannets. Representative still.

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Fig. 3. Inclusion of E-cadherin into stationary clusters requires cis-, trans-, and cytoplasmic interactions. Inclusion of E-cadherin into stationary clusters.

Failure of chromosome disassembly in mip120 null ovarian nurse cells

Fig. 3. Changes in the total EPS/Chl a ratio and bend interval of trichomes before and after the removal of polysaccharide from the BG11-cultured N. flagelliforme.

Volume 2, Issue 3, Pages (March 2008)

Presentation transcript:

Fig. 2. Analysis of morphology, pluripotency marker expression and transgene silencing in the colonies emerging during reprogramming. Analysis of morphology, pluripotency marker expression and transgene silencing in the colonies emerging during reprogramming. (A) Expression of SSEA-4 and RV-RFP (upper panel), TRA-1-60 and NANOG (middle panel) and TRA-1-60 and RV-RFP (lower panel) in the cell clusters/colonies on day 9 of reprogramming showing the initiation of pluripotency marker expression before the cells achieve hESC-like morphology and transgene silencing. (B) Expression of the pluripotency markers (SSEA-4, TRA-1-60 and NANOG) and RV-RFP silencing in the colonies on day 16 of reprogramming. The emerging hiPSC colonies showed characteristic hESC-like morphology and retroviral transgene silencing allowing their easy identification. (C) Higher magnification images of RV-RFP− hiPSC colonies showing their hESC-like morphology – flat appearance, defined boundary and high nuclear-to-cytoplasmic ratio. (D) Non-hESC like RFP+ colonies which lacked the expression of pluripotency markers on day 16. All images are at 10× magnification, unless otherwise indicated. The broken lines show the characteristic boundaries of the emerging hiPSC colonies on the feeder cells. Sumitha Prameela Bharathan et al. Biology Open 2017;6:100-108 © 2017. Published by The Company of Biologists Ltd