Effect of cGMP on in vitro fluid secretion rate, cation (Na+ and K+) concentration and transport rate by adult female A. aegypti MTs stimulated with a.

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Effect of cGMP on in vitro fluid secretion rate, cation (Na+ and K+) concentration and transport rate by adult female A. aegypti MTs stimulated with a variety of diuretic hormones. cGMP (100 nmol l−1) was applied to adult MTs stimulated with 100 nmol l−1 5-HT, 10 nmol l−1 RhoprDH (CRF), 50 nmol l−1 CDP or 25 nmol l−1 DromeDH31 (DH31). Effect of cGMP on in vitro fluid secretion rate, cation (Na+ and K+) concentration and transport rate by adult female A. aegypti MTs stimulated with a variety of diuretic hormones. cGMP (100 nmol l−1) was applied to adult MTs stimulated with 100 nmol l−1 5-HT, 10 nmol l−1RhoprDH (CRF), 50 nmol l−1 CDP or 25 nmol l−1DromeDH31 (DH31). (A) The tubule secretion assay was performed over a 60 min incubation period for the diuretic and cGMP, and 120 min for unstimulated controls. (B) Na+ and (C) K+ concentrations in the secreted fluid were measured using ion-selective microelectrodes (ISME), and the values were used to calculate the cation transport rate and ratio (D–F). Values are presented as means±s.e.m., n=7–18. Bars that are not significantly different from unstimulated controls are denoted with the same letter, as determined by a one-way ANOVA and Bonferroni post-test. Statistical significance between the two experimental treatments involving a specific diuretic factor alone or in combination with cGMP is denoted by an asterisk, as determined by an unpaired t-test. Farwa Sajadi et al. J Exp Biol 2018;221:jeb177089 © 2018. Published by The Company of Biologists Ltd