Nrf2−/− mice had higher TGF-β1 transcription and FN expression.

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Nrf2−/− mice had higher TGF-β1 transcription and FN expression. Nrf2−/− mice had higher TGF-β1 transcription and FN expression. The mRNA level of Nrf2, NQO1, and GST (A) and TGF-β1, FN, and collagen IV (B) was measured by qRT-PCR. The data presented are relative mRNA level normalized to β-actin mRNA level, and the value from the untreated Nrf2+/+ group was set as 1. *P < 0.05 untreated vs. STZ-treated; #P < 0.05 Nrf2+/+ vs. Nrf2−/−. Data are expressed as means ± SD (n = 8). The protein level of FN, NQO1, and GAPDH was measured by immunoblot analysis (C, upper panel). Each lane contained total proteins from the kidney of different individual mice. The band intensity was calculated and normalized to GAPDH (C, lower panel). The value from the untreated Nrf2+/+ group was set as 1. *P < 0.05 untreated vs. STZ-treated; #P < 0.05 Nrf2+/+ vs. Nrf2−/−. Data are expressed as means ± SD (n = 3). Tao Jiang et al. Diabetes 2010;59:850-860 ©2010 by American Diabetes Association