Dissociation of HP1α from H3K9me3 is critical to potentiation of steady-state ATM activity. Dissociation of HP1α from H3K9me3 is critical to potentiation.

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Dissociation of HP1α from H3K9me3 is critical to potentiation of steady-state ATM activity. Dissociation of HP1α from H3K9me3 is critical to potentiation of steady-state ATM activity. (A) Immunoprecipitation and Western blot analysis showing that mutants of HP1α (V23M and KW41/42AA) cannot bind H3K9me3. After knocking down the endogenous HP1α protein, the HA-tagged wild-type HP1α protein or the HA-tagged mutants of HP1α (V23M or KW41/42AA) were exogenously expressed in MCF7 cells. 500 μg of the lysates from these cells was immunoprecipitated with either normal IgG or anti-HP1α antibody and then analyzed by immunoblotting using the anti-H3K9me3 antibody or the anti-HA antibody. (B, C) Percentages of cells containing three or more large phospho-ATM (Ser 1981) foci. Columns and bars represent the mean of three independent experiments and SD, respectively. A total of 100 cells were examined for each cell type. (D) Immunofluorescence visualization of ATM phosphorylated on Ser 1981 and H3K9me3. Cells were stained with anti-phospho ATM (Ser 1981) (green) and anti-H3K9me3 (red) antibodies and DAPI (blue). In the “merge” panel, the red panel and green panel are merged to evaluate the localization of phospho-ATM (Ser 1981) (green) and H3K9me3 (red) in a single cell. Scale bar, 10 μm. (E) Schematic representation showing that expression levels of SYCE2 in somatic cells define the steady-state ATM activity by affecting HP1 localization. When the expression level of SYCE2 is low (left), HP1 is bound to H3K9me3, and the steady-state ATM activity is kept low. On the other hand, when the expression level of SYCE2 is high (right), direct binding of SYCE2 to HP1 insulates HP1 from H3K9me3, namely heterochromatin. As a result, HP1 will be distributed in euchromatin as well, which will potentiate the steady-state ATM activity and increase the levels of total DNA repair activity when DNA damage is induced exogenously. Noriko Hosoya et al. LSA 2018;1:e201800021 © 2018 Hosoya et al.