Folic acid increases apical junctional pMLCK localization in vivo

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Fig. 5. Folic acid increases apical junctional pMLCK localization in vivo. Folic acid increases apical junctional pMLCK localization in vivo. (A–B) Stage 7 chick embryos incubated for 2 h ex vivo with or without exogenous folic acid (100 µm) and immunofluorescently labeled with pMLCK antibody (red). (C) Junctional and medial fluorescent intensity was measured from neural epithelial images and the junctional/medial intensity ratios were calculated. A population graph depicting the percentage of cells with specific increments of intensity ratios demonstrates that the folic acid experimental group has the most cells in an increment range that is greater than the control group (arrows). (D–E) Representative images of cryosections through the neural epithelial of embryos incubated ex vivo with or without folic acid for 2 h and immunofluorescently labeled with a pMLCK antibody. (F) The fluorescent intensity along a 50 µm apical/basal line was measured from several images of cryosections similar to D–E and the mean intensity along the line was calculated. Control and folic acid treated embryos were compared and Student's t-test was performed between groups at each position along the 50 µm line. The P-value at each position was plotted simultaneously with the normalized intensity value to demonstrate regions with significant differences. Note that the intensities differ near the apical junctional complex. Jessica B. Martin et al. Biology Open 2019;8:bio041160 © 2019. Published by The Company of Biologists Ltd