Fig. 3. PLD2 and PARN overexpression affects PARN and PLD2 protein and gene expression. PLD2 and PARN overexpression affects PARN and PLD2 protein and.

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Fig. 3. PLD2 and PARN overexpression affects PARN and PLD2 protein and gene expression. PLD2 and PARN overexpression affects PARN and PLD2 protein and gene expression. (A) PARN overexpression of catalytically active or deadenylase-inactive (PARN-H377A) plasmids and their effect on PLD2 gene expression in COS-7 cells. (B) PLD2 overexpression of catalytically active or lipase-inactive (PLD2-K758R) plasmids and their effect of PARN gene expression in COS-7 cells. (C-H) Co-expression of PARN and PLD2 in two different cell lines: COS-7 (C,E,G) and MCF-7 (D,F,H). (C,D) Protein expression by western blot (actin was used as a gel loading control). Cells were left untransfected (mock) or transfected with PLD2 or PARN plasmids individually or in combination (PLD2+PARN). (E,F) PLD2 gene expression and (G,H) PARN gene expression, in either case detected by RT-qPCR. Data presented as bars are means+s.e.m. The difference between means was assessed by single-factor ANOVA. *P<0.05, **P<0.01, ***P<0.005, significant increase between samples and controls; #P<0.05, significant decrease between samples and controls. Taylor E. Miller, and Julian Gomez-Cambronero Biology Open 2017;6:176-186 © 2017. Published by The Company of Biologists Ltd