Proteomics of cerebral injury in a neonatal model of cardiopulmonary bypass with deep hypothermic circulatory arrest Amir M. Sheikh, MBBS, MRCS, Cindy.

Slides:

Advertisements

Similar presentations

Manuel J. Antunes, MD, PhD, DSc

Advertisements

Leukocyte filtration to decrease the number of adherent leukocytes in the cerebral microcirculation after a period of deep hypothermic circulatory arrest

Inhibition of microRNA-29c protects the brain in a rat model of prolonged hypothermic circulatory arrest Yongchao Wang, MD, Tianxiang Gu, MD, PhD, Enyi.

One-lung ventilation: For how long?

Urinary proteomics before and after extracorporeal circulation in patients with and without acute kidney injury Fabienne Aregger, MD, Christiane Pilop,

Expanding options to manage traumatic thoracic vascular injuries

The quest to optimize neurodevelopmental outcomes in neonatal arch reconstruction: The perfusion techniques we use and why we believe in them Richard.

Right ventricular hypertrophy with early dysfunction: A proteomics study in a neonatal model Amir M. Sheikh, MBBS, FRCS(C-Th), Cindy Barrett, MD, Nestor.

Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,

Protection of rat spinal cord from ischemia with dextrorphan and cycloheximide: Effects on necrosis and apoptosis Hiroyuki Kato, MD, PhDa c, Georgios.

Intermittent perfusion protects the brain during deep hypothermic circulatory arrest1 Stephen M Langley, Paul J Chai, MD, Sara E Miller, PhD, James R.

Derrick Y. Tam, MD, Stephen E. Fremes, MD, MSc, FRCSC, FACP, FACC

Erythropoietin protects the central nervous system during prolonged hypothermic circulatory arrest: An experimental study in a canine model Mitsuhiro.

Intrinsic cardiac stem cells are essential for regeneration

Comparison of neurologic outcome after deep hypothermic circulatory arrest with alpha- stat and pH-stat cardiopulmonary bypass in newborn pigs Margaret.

Definition of postoperative bleeding in children undergoing cardiac surgery with cardiopulmonary bypass: One size doesn't fit all David Faraoni, MD,

Inflammatory lung injury after cardiopulmonary bypass is attenuated by adenosine A2A receptor activation Turner C. Lisle, MD, Leo M. Gazoni, MD, Lucas.

Inhibition of microRNA-29c protects the brain in a rat model of prolonged hypothermic circulatory arrest Yongchao Wang, MD, Tianxiang Gu, MD, PhD, Enyi.

The lord of the rings Antonio Miceli, MD, PhD

Rodent brain slice model for the study of white matter injury

Does moderate hypothermia really carry less bleeding risk than deep hypothermia for circulatory arrest? A propensity-matched comparison in hemiarch replacement

Neurologic outcome after cardiopulmonary bypass with deep hypothermic circulatory arrest in rats: Description of a new model Bettina Jungwirth, MD, G.

Increasing duration of circulatory arrest, but not antegrade cerebral perfusion, prolongs postoperative recovery after neonatal cardiac surgery Selma.

Leukocyte filtration to decrease the number of adherent leukocytes in the cerebral microcirculation after a period of deep hypothermic circulatory arrest

Effects of carbamazepine on spinal cord ischemia

Ivan Ricardo Argueta-Morales, MD, Hamish M

John A. Elefteriades, MD, PhD (hon), Bulat A. Ziganshin, MD, PhD

Rohit K. Singal, MD, MSc, FRCSC, Rakesh C. Arora, MD, PhD, FRCSC

Innovation and science: The future of valve design

Tom Waterbury, MPS, CCP, LP, Thomas J

It's not “just a shunt” but sometimes it should be…

A first start for lung transplantation?

Get it right the first time

The Journal of Thoracic and Cardiovascular Surgery

Joseph A. Dearani, MD, Michael J. Ackerman, MD, PhD

Patient characteristics are important determinants of neurodevelopmental outcome at one year of age after neonatal and infant cardiac surgery J. William.

Commentary: Faster recovery after complex neonatal cardiac surgery

Effect of granulocyte-colony stimulating factor on expression of selected proteins involved in regulation of apoptosis in the brain of newborn piglets.

Should early extubation be the goal for children after congenital cardiac surgery? Kevin C. Harris, MD, MHSc, Spencer Holowachuk, BHK, Sandy Pitfield,

Left sinus of Valsalva aneurysm: Rare disease, rarer presentation

Primary pulmonary artery Rosai-Dorfman disease mimicking sarcoma

Instrumental variable methods in clinical research

Straight deep hypothermic circulatory arrest for cerebral protection during aortic arch surgery: Safe and effective Bulat A. Ziganshin, MD, Bijoy G.

Case reporter's notebook: The who, what, when, where, how, and why of extraction of a benign intracaval tumor Verdi J. DiSesa, MD, MBA The Journal of.

The Ross procedure: Time to reevaluate the guidelines

Cardiac myxoma: Simplifying a “complex” case

Histopathologic consequences of hyperglycemic cerebral ischemia during hypothermic cardiopulmonary bypass in pigs Brendan P Conroy, MD, Marjorie R Grafe,

Passing the torch The Journal of Thoracic and Cardiovascular Surgery

Effects of moderate versus deep hypothermic circulatory arrest and selective cerebral perfusion on cerebrospinal fluid proteomic profiles in a piglet.

One-lung ventilation: For how long?

James I. Fann, MD, John E. Connolly, MD

Deep hypothermic circulatory arrest during the arterial switch operation is associated with reduction in cerebral oxygen extraction but no increase in.

Nonintubated thoracoscopic surgery for pulmonary lesions in both lungs

Antegrade cerebral perfusion reduces apoptotic neuronal injury in a neonatal piglet model of cardiopulmonary bypass Valerie Y. Chock, MD, Gabriel Amir,

The Journal of Thoracic and Cardiovascular Surgery

Discussion The Journal of Thoracic and Cardiovascular Surgery

The future of cardiac surgery training: A survival guide

Apparently, size matters…in congenital heart disease and brain injury

Ventricular assistant in restrictive cardiomyopathy: Making the right connection Robert D.B. Jaquiss, MD The Journal of Thoracic and Cardiovascular Surgery

Between a rock and a hard place

Vinay Badhwar, MD, John S. Ikonomidis, MD, PhD, Jeffrey P. Jacobs, MD

“The more things change…”: The challenges ahead

Ryan R. Davies, MD The Journal of Thoracic and Cardiovascular Surgery

Apples remain apples NO matter what

Respect the aorta The Journal of Thoracic and Cardiovascular Surgery

Sunjay Kaushal, MD, PhD, Brody Wehman, MD

A randomized clinical trial of regional cerebral perfusion versus deep hypothermic circulatory arrest: Outcomes for infants with functional single ventricle

Did you like Terminator 3 better than Terminator 2

Left atrial to left ventricle bypass for mitral valve stenosis

A good chimney requires a good sweep

Presentation transcript:

Proteomics of cerebral injury in a neonatal model of cardiopulmonary bypass with deep hypothermic circulatory arrest Amir M. Sheikh, MBBS, MRCS, Cindy Barrett, MD, Nestor Villamizar, MD, Oscar Alzate, PhD, Sara Miller, PhD, John Shelburne, MD, PhD, Andrew Lodge, MD, Jeffrey Lawson, MD, PhD, James Jaggers, MD The Journal of Thoracic and Cardiovascular Surgery Volume 132, Issue 4, Pages 820-828.e2 (October 2006) DOI: 10.1016/j.jtcvs.2006.07.002 Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Example of a 2-dimensional differential gel electrophoresis of brain neocortex. The Cy2 (internal control) image is shown including identification numbers for the protein spots of interest. A typical 3-dimensional representation of a protein gel spot, as analyzed and displayed by DeCyder software, is shown below the gel, taking apoA1 as an example. The Cy5 (sham) protein image is on the left and the Cy3 (DHCA) image on the right. It can be seen there is a decrease of apoA1 peptide in the DHCA animal. The Journal of Thoracic and Cardiovascular Surgery 2006 132, 820-828.e2DOI: (10.1016/j.jtcvs.2006.07.002) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 All panels are of piglet neocortex. Panels A, C, and E are representative control samples. Panels B, D, F are representative experimental samples. Panels A and B are 100× magnification light micrographs (stained with hematoxylin and eosin) of neocortex; micron bar represents 50 μm. Panel A shows intact, normal neuropil, nuclei (N), and vasculature (V). Panel B shows evidence of swelling of the neuropil, including swollen and karyolytic nuclei, and pericellular and perivascular edema. Panels C and D are transmission electron micrographs at 3000× magnification; micron bar represents 1 μm. Panel C shows intact axons with normal mitochondria (M), as well as an intact plasma membrane (arrow). In contrast, in panel D the architecture is disrupted by swollen mitochondria and organelles. There is loss of plasma membrane, indicative of irreversible cell injury. Panels E and F are transmission electron micrographs at 5000× magnification; micron bar represents 1 μm. Panel E shows a normal nucleus (N), mitochondria (M), endoplasmic reticulum (ER), and an intact plasma membrane (PM). In contrast, panel F exhibits karyolysis, chromatin clumping, and edema. Mitochondria appear swollen. Flocculent deposits are present in mitochondria (arrow); this also denotes irreversible cell injury. DHCA, deep hypothermic circulatory arrest. The Journal of Thoracic and Cardiovascular Surgery 2006 132, 820-828.e2DOI: (10.1016/j.jtcvs.2006.07.002) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions