Protease digestion of seven purified MBP-CBS protein alleles.

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Protease digestion of seven purified MBP-CBS protein alleles. Protease digestion of seven purified MBP-CBS protein alleles. The conditions of the thermolysin digests are as in Figure 5, and the order of proteins from the top is that of heme incorporation, from highest to lowest. In each case the relevant doublet band of full-length CBS is indicated by the arrow head. The lower portion of the gel showing the cleaved MBP band is not shown. Dago Dimster-Denk et al. G3 2013;3:1619-1628 ©2013 by Genetics Society of America