Volume 53, Issue 4, Pages (April 1998)

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Volume 53, Issue 4, Pages 958-962 (April 1998) The Na+-K+-ATPase β1 subunit is associated with the HKα2 protein in the rat kidney Jeffrey A. Kraut, Jan Hiura, Jai M. Shin, Adam Smolka, George Sachs, David Scott Kidney International Volume 53, Issue 4, Pages 958-962 (April 1998) DOI: 10.1111/j.1523-1755.1998.00841.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 A typical immunoblot study of rat renal microsomal protein isolated with antibody AS 31.7 and probed with antibodies directed against HKα2 or Na+-K+-ATPase α1(N = 5). The HKα2 protein was isolated from 125 μg of renal microsomal protein from low K (lanes 3 and 4) or normal animals (lanes 5 and 6) using AS 31.7. Distinct bands were noted with low K and normal kidney probed with AS 31.7 but not with the antibody directed against Na+-K+-ATPase α1. On the other hand, a band was noted when purified renal Na+-K+-ATPase was probed with the Na+-K+-ATPase α1-specific antibody but not with the HKα2-specific antibody. Kidney International 1998 53, 958-962DOI: (10.1111/j.1523-1755.1998.00841.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 A typical immunoblot study of renal microsomal protein isolated with AS 31.7 and probed with HKβ1-specific antibody or Na+-K+-ATPase β1-specific antibody (N = 6). The HKα2 protein was isolated from 500 μg (lanes 5 and 6), 250 μg (lanes 7 and 8) and 125 μg (lanes 9 and 10) of renal microsomal protein using AS 31.7 and then probed with HKβ1-specific antibody (2B6) or Na+-K+-ATPase β1-specific antibody. Distinct bands were noted with the Na+-K+-ATPase β1 antibody at all concentrations of renal microsomal protein (lanes 6, 8, 10) that comigrated with Na+-K+-ATPase probed with this antibody (lane 4). No bands were detected at any concentration of renal microsomal protein probed with the HKβ1 antibody. Moreover, a band was detected with the G1 fraction of the stomach (purified H+-K+-ATPase) (lane 1) probed with the HKβ1 antibody but not when the G1 fraction was probed with Na+-K+-ATPase β1 antibody. Kidney International 1998 53, 958-962DOI: (10.1111/j.1523-1755.1998.00841.x) Copyright © 1998 International Society of Nephrology Terms and Conditions