Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen  Nabil Aziz, M.D., Jiri Novotny, Ph.D., Ivana Oborna, M.D., Ph.D., Helena Fingerova, Ph.D., Jana Brezinova, Ph.D., Magda Svobodova, M.A.  Fertility and Sterility  Volume 94, Issue 7, Pages 2604-2608 (December 2010) DOI: 10.1016/j.fertnstert.2010.03.022 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Dot plot chart displaying characteristics of forward scatter (FSc), which is roughly proportional to the diameter of the cell, and side scatter, which is proportional to cellular granularity. Each cell is represented by a dot, positioned on the X and Y scales according to the intensities detected for that cell. In gate R3 round cells heterogeneous group of cells are referred as “other round cells.” X = FSc; Y = side scatter; leu = leukocytes. Fertility and Sterility 2010 94, 2604-2608DOI: (10.1016/j.fertnstert.2010.03.022) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Box and whisker plot for the percentage contribution of the different semen cellular components to the overall intracellular H2O2 and peroxynitrite levels as measured with use of flow cytometry. The central “box” represents the distance between the first and third quartiles with the median between them marked with a diamond. LEU. = leukocytes; RC. = “other round cells”; DHR = dihydrorhodamine. Fertility and Sterility 2010 94, 2604-2608DOI: (10.1016/j.fertnstert.2010.03.022) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions