GLUT4 LXRE is required for downregulation of adipose GLUT4 mRNA during fasting and diet-induced obesity. GLUT4 LXRE is required for downregulation of adipose.

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GLUT4 LXRE is required for downregulation of adipose GLUT4 mRNA during fasting and diet-induced obesity. GLUT4 LXRE is required for downregulation of adipose GLUT4 mRNA during fasting and diet-induced obesity. C57BL/6 mice (2- to 3-month-old) mice were fed a 10% fat diet (control) or 60% high-fat diet (HFD) for 8 weeks. Fasted animals were animals fed the 10% fat diet that were deprived of food. A: Schematic drawing depicts the constructs used to generate transgenic animals. B: Total mRNA from perigonadal adipose tissue was isolated and used to determine mRNA levels of CAT, GLUT4, and actin. CAT and GLUT4 mRNA levels were normalized to actin, and the normalized mRNA levels were expressed as percent of the control mRNA for each experimental group. Data were analyzed using a two-tailed Student t test. *P < 0.05 for difference between experimental and control groups. Juston C. Weems et al. Diabetes 2012;61:1404-1414 ©2012 by American Diabetes Association