Ery-dependent pauses at XP(X) motifs are intensified by EF-P. Ery-dependent pauses at XP(X) motifs are intensified by EF-P. (A, B) Plots of pause strength in the presence of EF-P against pause strength in the absence of EF-P, for samples (A) without antibiotic or (B) with Ery. XP(P) motifs are indicated in orange, PP(X) motifs in yellow, and XP(X) motifs in blue. All other motifs are in gray. Most XP(X) motifs induce significant drug-dependent pauses in the presence of EF-P, but not in its absence. XP(P) and PP(X) motifs also behave differently in the presence of a drug, with pausing at XP(P) motifs being efficiently rescued by EF-P, whereas PP(X) motifs induce greater pausing in the presence of EF-P. Britta Seip et al. LSA 2018;1:e201800148 © 2018 Seip et al.