Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization Julie M Clyde, Ph.D., Jan E Hogg, M.Sc., Anthony J Rutherford,

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Presentation transcript:

Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization Julie M Clyde, Ph.D., Jan E Hogg, M.Sc., Anthony J Rutherford, M.B.B.S., Helen M Picton, Ph.D. Fertility and Sterility Volume 80, Issue 4, Pages 1003-1011 (October 2003) DOI: 10.1016/S0015-0282(03)01158-0

FIGURE 1 M-FISH pseudocolor image of a normal oocyte metaphase (23,X) with (a) inverted DAPI stained chromosomes inset and (b) corresponding karyotype. (c and d), Hyperploid oocyte 24,X+18 showing nondisjunction, with the additional chromosome 18 indicated by an arrow. Pseudocolor image of (e) sperm prematurely condensed chromosomes in an injected oocyte (f) karyotyped as 23,X. All chromosomes are shown in M-FISH option to demonstrate probe specificity (flat color). Clyde. M-FISH in metaphase II oocytes.Fertil Steril 2003. Fertility and Sterility 2003 80, 1003-1011DOI: (10.1016/S0015-0282(03)01158-0)