The SIM domain of RAD51 is required for HR

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The SIM domain of RAD51 is required for HR The SIM domain of RAD51 is required for HR. (A) HR activity of RAD51-depleted cells (siRAD51), expressing NLS (vector), NLS-RAD51-WT, NLS-RAD51-V2645K or NLS-RAD51-AAAS, was analyzed using the DR-GFP assay. The SIM domain of RAD51 is required for HR. (A) HR activity of RAD51-depleted cells (siRAD51), expressing NLS (vector), NLS-RAD51-WT, NLS-RAD51-V2645K or NLS-RAD51-AAAS, was analyzed using the DR-GFP assay. Columns represent the mean of the proportions of GFP-positive cells from four independent experiments. Error bars represent s.d. (B) Expression of NLS-RAD51-WT, NLS-RAD51-V2645K or NLS-RAD51-AAAS in RAD51-depleted cells (siRAD51) was confirmed by an immunoblot analysis using anti-RAD51 antibodies. Hiroki Shima et al. J Cell Sci 2013;126:5284-5292 © 2013. Published by The Company of Biologists Ltd