Sufu is required for the stabilization of Gli2 and Gli3 proteins in vivo. Sufu is required for the stabilization of Gli2 and Gli3 proteins in vivo. (A)

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Sufu is required for the stabilization of Gli2 and Gli3 proteins in vivo. Sufu is required for the stabilization of Gli2 and Gli3 proteins in vivo. (A) Gross morphology of wild-type and Sufu−/− embryos at E9.5. (B) Immunoblot showing that levels of Gli2 and Gli3 proteins are significantly reduced in Sufu−/− embryos. The relative intensity of Gli2FL, Gli3FL and Gli3Rep was quantified by NIH image software and is shown above each bar in the righthand graph. (C) Overexpression of Sufu stabilizes Gli2 and Gli3 proteins in HEK293 cells. Chengbing Wang et al. Development 2010;137:2001-2009 © 2010.