Gene expression signature of NK cells in NSG hL-7xhIL-15 humanized mice. Gene expression signature of NK cells in NSG hL-7xhIL-15 humanized mice. RNA sequencing.

Slides:

Advertisements

Similar presentations

( ) 2.2 FC autism control FGR1OP2. ( ) 1.4 FC autism control SMAD2.

Advertisements

SP receptor neurokinin 1 receptor (NK1R) is expressed in adipocytes and preadipocytes, and SP interferes with differentiation. 3T3-L1 preadipocytes were.

Anifrolumab inhibits cytokine production, plasmacytoid dendritic cell (pDC) activation and the type I (interferon gene signature) IFN gene signature. Anifrolumab.

محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)

(A) Heat map showing expression changes during in vitro differentiation of nscs to astrocytes. (A) Heat map showing expression changes during in vitro.

Scratching the Surface of Immunotherapeutic Targets in Neuroblastoma

RNA-seq analysis of iWAT and eWAT.

Scratching the Surface of Immunotherapeutic Targets in Neuroblastoma

Type 17 immunity is increased in STAT1−/− mice.

IRF4 regulates cytokine receptor expression in CD8+ T cells.

Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent and cycling LGR5+ CRC cells Dual LGR5 and KI67 knock‐in PDOs enable separation of quiescent.

K107R induces gene expression in an adipocyte-autonomous manner.

Effects of SC144 on in vivo ovarian tumor.

Functions of myeloid cells.

HCMV jogs the ‘memory’ of NK cells in HBV

Expression of NCoR1∆ID in PAX8−/− mice does not reverse repression of T3-positive target genes. qPCR on the indicated pituitary (A) and neuronal (B) genes.

Correlation of mouse gene expression with bacterial gene expression.

Splenic CD169+ macrophages express a unique gene profile.

Evaluation of re-annotation for non-melanogaster Drosophila species.

Immune cell populations, activation, and NKG2D and H60a expression in the spleen of untreated and antibiotic-treated Klrk1+/+ and Klrk1−/− NOD mice. Immune.

Human TCM have potent effector functions, home to skin, and induce dermatitis in human engrafted mice. Human TCMhave potent effector functions, home to.

Validation of RNA transcription profile differential expression using real-time quantitative PCR. Relative gene expression in cases compared with controls.

Marker genes are selected for mouse brain cell types and used to estimate cell type profiles. Marker genes are selected for mouse brain cell types and.

qRT-PCR validation of differential expression.

Changes in protein expression during distinct stages of NK cell differentiation. Changes in protein expression during distinct stages of NK cell differentiation.

Relative expression levels of known mRNA selected as points of reference. Relative expression levels of known mRNA selected as points of reference. mRNA.

Knockdown efficiency of different shRNAs.

Production of Mapk14 and Mapk7 KO cells.

Distribution of splenic T cells, B cells, and NK cells in NSG hL-7xhIL-15 humanized mice. Distribution of splenic T cells, B cells, and NK cells in NSG.

FACT correlates with active gene expression.

Changes in mutation rate or protein abundance are not observed in HATs when comparing rho+ to rho0 cells. Changes in mutation rate or protein abundance.

Development of human T-cell subsets in NSG hL-7xhIL-15 humanized mice.

CiCo relation to linear transcripts.

LLC cells exhibit a blunted response to IFNγ in vitro and in vivo compared with CMT167. LLC cells exhibit a blunted response to IFNγ in vitro and in vivo.

Therapy model: CD34+CD33Del cells resist CD33-targeted immunotherapy.

Summary of RNA FISH quantifications.

Schematic representation of a transcriptomic evaluation approach.

Increased reconstitution of human NK cells in NSG hIL-7xhIL-15 humanized mice. Increased reconstitution of human NK cells in NSG hIL-7xhIL-15 humanized.

BTG2 regulates gluconeogenic gene expression in diabetic mouse models

Transcriptomic profiling of host cells during ZIKV infection.

Comparison of conventional NSG and hIL-7 expressing NSG humanized mice

BAP1 is required for homeostatic and antigen-driven expansion of peripheral T cells. BAP1 is required for homeostatic and antigen-driven expansion of peripheral.

IRS-1 and -2 expression in four mesangial cell lines from different D-NOD (A and B) and ND-NOD (C and D) mice. IRS-1 and -2 expression in four mesangial.

CD8α+, CD8α−, and MoDCs from NOD.hCD205 mice express hCD205.

CREB1 promotes the induction of endogenous ERα target genes.

The endogenous IGRP gene is selectively expressed in βTC-3 cells and not in Y1 cells. βTC-3 RNA (50 μg) and Y1 RNA (50 μg) were annealed to labeled oligonucleotide.

MK-8628 modulates transcriptional signatures of activated T lymphocytes. MK-8628 modulates transcriptional signatures of activated T lymphocytes. Gene.

LG NK cells appear to be conventional in phenotype.

CD8 T cell memory alters the immune profile in enhanced HLH without altering viral load. CD8 T cell memory alters the immune profile in enhanced HLH without.

Human IL-10 neutralization results in inhibited expression of intragraft Treg function genes and production of suppressive cytokine IL-10. Human IL-10.

Overexpression of IL-27 upregulates expression of multiple IR by T cells in vivo. Overexpression of IL-27 upregulates expression of multiple IR by T cells.

NK Cells Fighting Cancer

Daniel L. Roden, Shane T. Grey Kidney International

Conversion of CD11bhighCD27high NK cells into MDSCs leads to CD11bhighCD27high and CD11bhighCD27low NK cell reduction. Conversion of CD11bhighCD27high.

Fig. 4 Bcl11b regulates expression of key Treg cell genes while suppressing inflammatory and innate genes. Bcl11b regulates expression of key Treg cell.

PD-1 inhibition stimulates the proliferation and cytokine secretion of exhausted/senescent CD8+ T cells in vitro. PD-1 inhibition stimulates the proliferation.

Constitutive characteristics in HL-mice and HL-HPS-mice.

Induction of cytotoxic activity in humanized SCC3 tumor-bearing mice treated with anti-PD-1 antibody. Induction of cytotoxic activity in humanized SCC3.

Increase in proliferation and activation of immune cells in peripheral blood after NKTR-214 treatment. Increase in proliferation and activation of immune.

Transcripts enriched and depleted in NB TICs compared with SKPs and other tumor tissues. Transcripts enriched and depleted in NB TICs compared with SKPs.

Tregs preferentially regulate TH2 cytokines in skin.

Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus tissues after stimulation in vivo with poly(I:C). Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus.

Defining the eTME genes.

Expression of chemokine receptors in A-498 cell line.

Increased tumorigenic activities of TIM-3–expressing RCC cells.

Highly metastatic PDAC cells have a unique gene signature, which is not preserved in metastases but predicts poor patient outcome. Highly metastatic PDAC.

Transcriptome profiling of PD-L1 antibody–treated macrophages showed inflammatory phenotype, increased survival and proliferation, and decreased apoptosis.

ADAGE extracted features that represented sequence differences between strains. ADAGE extracted features that represented sequence differences between.

Differences in tumour cell metabolism and cytokine/chemokine expression among the three HCC subtypes. Differences in tumour cell metabolism and cytokine/chemokine.

Cxxc1-deficient TH17 cells exhibit a Treg cell–like expression profile

Presentation transcript:

Gene expression signature of NK cells in NSG hL-7xhIL-15 humanized mice. Gene expression signature of NK cells in NSG hL-7xhIL-15 humanized mice. RNA sequencing was performed using RNA extracted from hCD56+ splenic NK cells of conventional NSG (NSG, n = 2) and hIL-7xhIL-15 KI NSG (hIL7xhIL-15 KI NSG, n = 6) humanized mice. (A) Differentially expressing genes are shown. Gene expression profiles of human NK cells of humanized mice were compared with those from NK cells recovered from human PBMCs. (B) Expression of genes related to cytokine and cytotoxicity are shown. Masashi Matsuda et al. LSA 2019;2:e201800195 © 2019 Matsuda et al.