Circulating microRNAs as potential biomarkers for endometriosis SiHyun Cho, M.D., Levent Mutlu, M.D., Olga Grechukhina, M.D., Hugh S. Taylor, M.D.  Fertility and Sterility  Volume 103, Issue 5, Pages 1252-1260.e1 (May 2015) DOI: 10.1016/j.fertnstert.2015.02.013 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 MicroRNAs differentially expressed in the sera of endometriosis patients and controls. (A–D) The expression levels of let-7b and mir-135a were statistically significantly lower in patients with endometriosis than in controls, and let-7d and 7f showed a trend toward decreased expression. Data are represented by box-and-whisker plots. Boxes indicate the 25th and 75th percentiles, with a solid line within the box showing the median value. Fertility and Sterility 2015 103, 1252-1260.e1DOI: (10.1016/j.fertnstert.2015.02.013) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Expression levels of miRNA in serum from endometriosis patients and controls according to the menstrual cycle. During the proliferative phase, (A) let-7b, (B) let-7c, (C) let-7d, and (D) let-7e were statistically significantly down-regulated in endometriosis patients compared with controls. (E) The expression levels of let-7f showed a trend toward down-regulation. (F) During the secretory phase, mir-135a was statistically significantly down-regulated in patients with endometriosis compared with controls. Data are represented by box-and-whisker plots. Boxes indicate the 25th and 75th percentiles, with a solid line within the box showing the median value. Fertility and Sterility 2015 103, 1252-1260.e1DOI: (10.1016/j.fertnstert.2015.02.013) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Receiver operating characteristic (ROC) curves of different miRNAs during the proliferative phase. (A) Let-7d showed the highest AUC of 0.905. (B) When the combination of the serum miRNA expression levels during the proliferative phase was used with a logistic regression model, the combination of serum let-7b, let-7d, and let-7f showed the highest AUC value of 0.929. Fertility and Sterility 2015 103, 1252-1260.e1DOI: (10.1016/j.fertnstert.2015.02.013) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Correlations between let-7b expression levels and serum CA-125 levels. A significant negative correlation was noted (r = −0.333). Fertility and Sterility 2015 103, 1252-1260.e1DOI: (10.1016/j.fertnstert.2015.02.013) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions