ABCG2-K86M shows a markedly reduced surface expression.

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ABCG2-K86M shows a markedly reduced surface expression. ABCG2-K86M shows a markedly reduced surface expression. (A) Surface expression of ABCG2 in the stably transfected HEK293 cells were determined by flow cytometry. The cells were stained with PE-conjugated anti-ABCG2 antibody directed against the extracellular domain of ABCG2. Dead cells were detected and omitted by staining with Propidium Iodide. An IgG isotype control antibody was used to detect unspecific binding. The histogram represents the surface expression (mean fluorescence) normalized to ABCG2-wt-MYC (ABCG2-wt-cmyc) (n=3). (B) HEK293 cells stably expressing ABCG2-K86M or ABCG2-wt-MYC + ABCG2-K86M-HA were analyzed by immunocytochemistry to detect localization of the transporter. The cells were stained with anti-ABCG2 or anti-HA antibody and a secondary Alexa 488 antibody. The samples were analyzed using an Axiovert 200 epifluorescence microscope. Ulla Henriksen et al. J Cell Sci 2005;118:1417-1426 © The Company of Biologists Limited 2005