Association of maternal and fetal MTHFR A1298C polymorphism with the risk of pregnancy loss: a study of an Indian population and a meta-analysis  Rohini R. Nair, M.Sc., Anuradha Khanna, M.D., Rajender Singh, Ph.D., Kiran Singh, Ph.D.  Fertility and Sterility  Volume 99, Issue 5, Pages 1311-1318.e4 (April 2013) DOI: 10.1016/j.fertnstert.2012.12.027 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Forest plot showing the odds ratio of each study and the pooled estimate, and (B) funnel plot of standard error by log odds ratio showing analysis of publication bias of studies in recurrent pregnancy loss. (C) Forest plot showing the odds ratio of each study and the pooled estimate, and (D) funnel plot of standard error by log odds ratio showing analysis of publication bias of studies in spontaneously aborted embryos. Fertility and Sterility 2013 99, 1311-1318.e4DOI: (10.1016/j.fertnstert.2012.12.027) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 MTHFR A1298C mutation was detected by polymerase chain reaction–restriction-fragment-length polymorphism. The 163 bp was digested with MboII. The A allele is cut by the enzyme and yields 56, 31, 30, 28, and 18 bp, whereas the C allele yields 84, 31, 30, and 18 bp products. Lane 1: 100 bp ladder (New England Biolabs); lane 2: AA homozygous; lanes 3 and 4: AC heterozygous; lane 5: CC homozygous. Fertility and Sterility 2013 99, 1311-1318.e4DOI: (10.1016/j.fertnstert.2012.12.027) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Flow diagram of the literature search. RPL = recurrent pregnancy loss. Fertility and Sterility 2013 99, 1311-1318.e4DOI: (10.1016/j.fertnstert.2012.12.027) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions