Single MTs can be resolved in subsections of SBF-SEM image stacks.

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Single MTs can be resolved in subsections of SBF-SEM image stacks. Single MTs can be resolved in subsections of SBF-SEM image stacks. (A) Overview of a spindle pole from a HeLa cell at late prometaphase. Astral MTs are rendered in light green, other MTs in darker green and centrioles are rendered in blue. A 3D rendering of slices 86–104 is shown on the left. Slice 92 is shown in the second panel followed by the indicated zooms of the SEM image and segmentation (4× equals a window size of 4.6 μm, 2× equals a window size of 1.15 μm). (B) 3D rendering of example kinetochores and their associated K-fibers (green). (C) Quantification of segmented MTs that terminate at kinetochores. Four datasets are shown from control cells, indicating the variability in detection. Spots are individual kinetochores, bars indicate mean±s.d. (D) A plot of kinetochore microtubules counted at a given kinetochore versus the number at the sister kinetochore. Overlapping points are highlighted by color as indicated. Faye M. Nixon et al. J Cell Sci 2017;130:1845-1855 © 2017. Published by The Company of Biologists Ltd