Quality and functional aspects of sperm retrieved through assisted ejaculation in men with spinal cord injury Adriana Ester Restelli, M.Sc., Ricardo.

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Presentation transcript:

Quality and functional aspects of sperm retrieved through assisted ejaculation in men with spinal cord injury Adriana Ester Restelli, M.Sc., Ricardo Pimenta Bertolla, D.V.M., Ph.D., Deborah Montagnini Spaine, Ph.D., Ari Miotto, M.D., Milton Borrelli, M.D., Agnaldo Pereira Cedenho, M.D., Ph.D. Fertility and Sterility Volume 91, Issue 3, Pages 819-825 (March 2009) DOI: 10.1016/j.fertnstert.2007.12.060 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Photomicrograph of sperm labeled for apoptotic DNA fragmentation by using a colorimetric terminal deoxynucleotidyl transferase–mediated dUTP nick end–labeling kit. Sperm with DNA fragmentation are stained brown, and sperm without DNA fragmentation are labeled green. Fertility and Sterility 2009 91, 819-825DOI: (10.1016/j.fertnstert.2007.12.060) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Photomicrograph of sperm evaluated for mitochondrial activity. Brown areas in the midpiece demonstrate active mitochondria, and areas without staining demonstrate inactive mitochondria. ∗Midpiece region without active mitochondria. Fertility and Sterility 2009 91, 819-825DOI: (10.1016/j.fertnstert.2007.12.060) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions