Genetics of Brain Development and Hydrocephalus

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Presentation transcript:

Genetics of Brain Development and Hydrocephalus Biomedical RaMP Final Presentation April 19, 2016 Genetics of Brain Development and Hydrocephalus Scott Emmert Mentor/PI: June Goto, PhD

The Research Mangano Research Lab, Department of Neurosurgery, CCHMC Dr. June Goto: PI, Research Instructor, and Neurobiologist Pediatric hydrocephalus – “water on the brain” New mutant gene - “progressive hydrocephaly, prh” Rat-prh project to study role of this gene in brain development

What? DNA Work Tissue Culture CRISPR/cas9 genome editing Preparing/splitting cells Plasmid preparation Lipofection Bacteria transformation Electroporation Gel electrophoresis DNA Sequencing & Genotyping Core PCR

Positive sequencing samples What? - Timeline Positive sequencing samples Transfection Rat work, surgery

So What? Sense of fulfillment Lab-to-life skills Gradually making a difference in lives of pediatric hydrocephalus patients Lab-to-life skills Accountability Collaboration Experiential learning Self-efficacy

Now What? Continuation of research into summer through SURF Further pursue rat-prh project Engage research as an application of my coursework Self-discovery Completely new perspective on biomedical research Career possibilities Physician-scientist

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