AIP1 localizes in the apical region of preclusters and clusters. AIP1 localizes in the apical region of preclusters and clusters. (A-A″′) Low magnification view of distribution patterns of AIP1-GFP, F-actin and Arm in a larval eye disc. The yellow dotted line marks the position of the cross-section, which is shown beneath each panel. Arrow points to the MF. (B-C″′) High magnification views showing that AIP1 is enriched together with F-actin underlying the AJs of preclusters (B-B″′) and that AIP1 colocalizes with F-actin in the AJ region of more mature clusters (C-C″′). Cofilin displays a diffuse staining pattern in the apical plane and does not show enrichment in the clusters or preclusters (see supplementary material Fig. S5B-B″). Scale bars: 10 μm in A-A″′; 5 μm in B-C″′. Dandan Chu et al. Development 2012;139:3561-3571 © 2012.