RIPA-based buffer system Harsh-conditioned system

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RIPA-based buffer system Harsh-conditioned system Supplementary Fig. S5 a 2 3 5a 5b 5c 6 7 8 10 13+Uev1a E2 (UbcH) + + + + + + + + + + + + + + + + + + + + FLAG-Ub + + + + + + + + + + + + + + + + + + + + E1 + + + + + + + + + + GST + + + + + + + + + + GST-ZNF313-WT GST-ZNF313-Ub(n)  GST-ZNF313 (Expected size) 170 - 72 - 55 - IB: anti-FLAG b 170 - 72 - 55 - PBS 2 5a 5b 5c 6 E2 (UbcH) + + + + + + + + + + + + + + + + + + FLAG-Ub + E1 + + + + + + GST-ZNF313-WT + + + + + + GST-ZNF313 RING-MT + + + + + + GST-ZNF313 ΔUIM GST-ZNF313-Ub(poly)  GST-ZNF313 (Expected size)  GST-ZNF313-Ub(mono) IB: anti-FLAG c 100 - 55 - 35 - WCL HeLa - + + - - + - + + HA-Ub - - + siZNF313-1 RIPA-based buffer system Harsh-conditioned system IP: p21WAF1 IB: HA IB: p21WAF1 170 - ZNF313 p21WAF1 HA Tubulin