Wild-type NSD3 is required for the blockade of differentiation in BRD4–NUT-expressing NMC cells. Wild-type NSD3 is required for the blockade of differentiation.

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Wild-type NSD3 is required for the blockade of differentiation in BRD4–NUT-expressing NMC cells. Wild-type NSD3 is required for the blockade of differentiation in BRD4–NUT-expressing NMC cells. A, immunoblots of BRD4–NUT-positive NMC cell lines TC-797, PER-403, and 8645 120 hours following transfection with control and NSD3 siRNAs stained with the terminal squamous differentiation marker involucrin, using GAPDH as loading control. B, representative photomicrographs of TC-797s 120 hours following transfection with either control or NSD3 siRNAs stained either with H&E for morphology, or involucrin IHC. All photographs are of identical magnification (×400). C, qRT-PCR of NSD3 levels 24 hours following transfection of control or NSD3 siRNAs. Primers were either 5′ of the breakpoint (NSD3–5′ primers), or 3′ of the breakpoint (NSD3-3′ primers) with NUT. Results are of a single biologic replicate performed in triplicate. Error bars, the mean ± SD of the triplicate wells. D, proliferation assay (Ki-67 fraction) comparing BRD4–NUT-positive TC-797, 8645, and PER-403 NMC cells transfected with control and NSD3–6 siRNAs. Three hundred cells were counted per cell block. E, 797TRex cells induced to express FLAG-tagged NLS-ET domain of BRD4 for 120 hours. Immunoblot was performed with anti-involucrin (Inv), anti-FLAG, or anti-GAPDH (left). Cell block preparations were H&E stained, or subjected to involucrin IHC (right). All photographs are of identical magnification (×400). F, cell viability assay (CellTiter-Glo) of 797TRex, 293TRex, and U2OSTRex cells induced to express FLAG-tagged NLS-ET domain for 120 hours. Results are the average of three biologic replicates, each performed in quadruplicate and normalized to the negative control (ethanol vehicle control) for each cell line. Error bars, the mean ± SD of the three biologic replicates. Immunoblot demonstrating NLS-FLAG-ET expression was stained with anti-FLAG or anti-GAPDH (right). Christopher A. French et al. Cancer Discovery 2014;4:928-941 ©2014 by American Association for Cancer Research