Cardiomyocytes cultured on microgroove-patterned structural color hydrogel films. Cardiomyocytes cultured on microgroove-patterned structural color hydrogel.

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Cardiomyocytes cultured on microgroove-patterned structural color hydrogel films. Cardiomyocytes cultured on microgroove-patterned structural color hydrogel films. (A) Schematic diagram of the generation process of the microgroove-patterned hydrogel films. (B) Fluorescent images of the cardiomyocytes cultured on the surface of the structural color hydrogel films with different concave side and convex side values, from left to right, are 20 and 30 μm, 40 and 30 μm, and 60 and 30 μm, respectively. (C) Confocal laser scanning microscopy (CLSM) images of the anisotropic laminar cardiomyocyte tissues on the surface of the microgroove-patterned inverse opal–structured hydrogel film. (D) Orientation angle frequency distribution of the cardiomyocytes on differently patterned substrates after 6 days of culture. Error bars represent SD. (E) Beating characterization of the cardiomyocytes on different patterned substrate. These dates were the average values of each day (10 min each time and five times every day). Scale bars, 20 μm (B) and 100 μm (C). Fanfan Fu et al. Sci. Robotics 2018;3:eaar8580 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works