A small N-terminal subfragment of fibrillin-1 inhibits osteoclastogenesis. A small N-terminal subfragment of fibrillin-1 inhibits osteoclastogenesis. (A)

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Peyman Hadji, Robert Coleman, Michael Gnant

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A small N-terminal subfragment of fibrillin-1 inhibits osteoclastogenesis. A small N-terminal subfragment of fibrillin-1 inhibits osteoclastogenesis. (A) Schematic of recombinant N-terminal (rFBN1-N) and C-terminal (rFBN1-C) halves of fibrillin-1, and recombinant sub-fragments of rFBN1-N – rF23, rF51, rF1F and rF18. (B–D) Bone marrow cells (white bars) and RAW 264.7 (black bars) were cultured for 5 days untreated (negative control, NC), treated with RANKL only (50 ng/ml, positive control, PC) or treated with RANKL and 50 µg/ml of soluble fibrillin-1 fragments rFBN1-N, rFBN1-C, rF23, rF51, rF1F or rF18. (B) Average numbers of osteoclasts formed under the indicated conditions. Data are means ± s.e.m., n = 3–8 experiments; *P<0.05, **P<0.01, ***P<0.001 compared with PC assessed by Student's t-test. (C,D) mRNA was isolated and the expression of cathepsin K (Ctsk, C) and Dcstamp (D) was quantified. Data are means ± s.e.m., n = 3–9 experiments; *P<0.05, ***P<0.001, compared with PC assessed by Student's t-test. Kerstin Tiedemann et al. J Cell Sci 2013;126:4187-4194 © 2013. Published by The Company of Biologists Ltd