Erik J. Saude, MD, PhD, Christopher D

Slides:

Advertisements

Similar presentations

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,

Advertisements

A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay Jing Lin, PhD, Francesca M. Bruni,

Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation So Ri Kim, MD, PhD, Dong Im Kim, MS, Mi.

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

What drives prescription patterns in pediatric asthma management?

Tetanus immunity after diphtheria, tetanus toxoids, and acellular pertussis vaccination in children with clinically stable HIV infection Howard M. Rosenblatt,

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Neighborhood poverty, urban residence, race/ethnicity, and asthma: Rethinking the inner-city asthma epidemic Corinne A. Keet, MD, PhD, Meredith C. McCormack,

Risk of an asthma exacerbation after bariatric surgery in adults

Ventilation defect percent in helium-3 magnetic resonance imaging as a biomarker of severe outcomes in asthma David G. Mummy, MS, Stanley J. Kruger,

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD

Mauro Maniscalco, MD, PhD, Debora Paris, PhD, Dominique J

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Ann-Marie M. Schoos, MD, PhD, Jacob D

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD

The effects of calcitriol treatment in glucocorticoid-resistant asthma

Is 9 more than 2 also in allergic airway inflammation?

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Analysis of basophil activation in patients with aspirin-exacerbated respiratory disease Chihiro Mitsui, MD, Keiichi Kajiwara, BSc, Emiko Ono, MD, PhD,

Airway hyperresponsiveness to mannitol and methacholine and exhaled nitric oxide: A random-sample population study Asger Sverrild, MD, Celeste Porsbjerg,

Profile of eicosanoid generation in aspirin-intolerant asthma and anaphylaxis assessed by new biomarkers Noritaka Higashi, MD, PhD, Haruhisa Mita, PhD,

Correction Journal of Allergy and Clinical Immunology

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

High-resolution metabolomics to identify urine biomarkers in corticosteroid-resistant asthmatic children Youngja H. Park, PhD, Anne M. Fitzpatrick, PhD,

Gut microbiome variations during hematopoietic stem cell transplant in severe combined immunodeficiency Jonathan P. Lane, MBBS, Christopher J. Stewart,

A network-based analysis of the late-phase reaction of the skin

Elina Jerschow, MD, MSc, Robert Y. Lin, MD, MSc, Moira M

Peter M. Wolfgram, MD, David B. Allen, MD

Etiology of asthma exacerbations

William J. Calhoun, MD, Tmirah Haselkorn, PhD, Dave P

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Peanut epitopes for IgE and IgG4 in peanut-sensitized children in relation to severity of peanut allergy Annebeth E. Flinterman, MD, Edward F. Knol,

Age-dependent effect of ambient ozone on emergency asthma hospitalizations in Hong Kong Yuanrong Dai, MD, Hong Qiu, PhD, Shengzhi Sun, PhD, Yang Yang,

Serum tryptase levels in atopic and nonatopic children

Staphylococcus aureus colonization is associated with wheeze and asthma among US children and young adults Meghan F. Davis, PhD, Roger D. Peng, PhD,

Bruce D. Miller, MD, Beatrice L

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Karen C. Dannemiller, PhD, Janneane F. Gent, PhD, Brian P

A cluster-randomized trial shows telephone peer coaching for parents reduces children's asthma morbidity Jane M. Garbutt, MB, ChB, Yan Yan, MD, PhD,

Altered metabolic profile in patients with IgE to galactose-alpha-1,3-galactose following in vivo food challenge John W. Steinke, PhD, Shawna L. Pochan,

Effect of ingested H1 antihistamines on methacholine challenge

Airway hyperresponsiveness to mannitol and methacholine and exhaled nitric oxide: A random-sample population study Asger Sverrild, MD, Celeste Porsbjerg,

What is an “eosinophilic phenotype” of asthma?

Increased sputum endotoxin levels are associated with an impaired lung function response to oral steroids in asthmatic patients Charles McSharry, PhD,

Immune responses to mosquito saliva in 14 individuals with acute systemic allergic reactions to mosquito bites Zhikang Peng, MD, Andrew N. Beckett, BSc,

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Nasal AMP and histamine challenge within and outside the pollen season in patients with seasonal allergic rhinitis Sriram Vaidyanathan, MBBS, Peter Williamson,

Advances in the approach to the patient with food allergy

Comparison of rhinovirus antibody titers in children with asthma exacerbations and species-specific rhinovirus infection Jua Iwasaki, BSc (Hons), Wendy-Anne.

Anne M. Fitzpatrick, PhD, Youngja Park, PhD, Lou Ann S

Martin Brasholt, MD, Florent Baty, PhD, Hans Bisgaard, MD, DMSci

Change in visual analog scale score in a pragmatic randomized cluster trial of allergic rhinitis Philippe-Jean Bousquet, MD, Christophe Combescure, PhD,

Fluctuation phenotyping based on daily fraction of exhaled nitric oxide values in asthmatic children Georgette Stern, MSc, Johan de Jongste, MD, Ralf.

Jessica H. Savage, MD, MHS, Elizabeth C. Matsui, MD, MHS, Robert A

Colin W. Summers, MSc, Richard S. Pumphrey, FRCPath, Charlotte N

Tetanus immunity after diphtheria, tetanus toxoids, and acellular pertussis vaccination in children with clinically stable HIV infection Howard M. Rosenblatt,

Päivi M. Salo, PhD, Samuel J. Arbes, DDS, MPH, PhD, Patrick W

Macrolide antibiotics and asthma treatment

Bo L. K. Chawes, MD, Matthew J

Asthma: The past, future, environment, and costs

Daniel Menzies, MBChB, Arun Nair, MBBS, Karen T

Negative affect, medication adherence, and asthma control in children

Effect of codeine on objective measurement of cough in chronic obstructive pulmonary disease Jaclyn Smith, MD, PhD, Emily Owen, MPhil, John Earis, MD,

Endogenous bradykinin and B1-B5 during angiotensin-converting enzyme inhibitor– associated angioedema Scott A. Hubers, MD, Kevin Kohm, MS, Shouzuo Wei,

Natural history of cow’s milk allergy

Neighborhood poverty, urban residence, race/ethnicity, and asthma: Rethinking the inner-city asthma epidemic Corinne A. Keet, MD, PhD, Meredith C. McCormack,

Analysis of basophil activation in patients with aspirin-exacerbated respiratory disease Chihiro Mitsui, MD, Keiichi Kajiwara, BSc, Emiko Ono, MD, PhD,

Urinary tetranor-PGDM concentrations in aspirin-intolerant asthma and anaphylaxis Noritaka Higashi, MD, PhD, Haruhisa Mita, PhD, Hiromichi Yamaguchi,

Presentation transcript:

Metabolomic profiling of asthma: Diagnostic utility of urine nuclear magnetic resonance spectroscopy Erik J. Saude, MD, PhD, Christopher D. Skappak, Shana Regush, BSc, Kim Cook, RN, Amos Ben-Zvi, PEng, PhD, Allan Becker, MD, FRCPC, Redwan Moqbel, PhD, FRCPath, Brian D. Sykes, PhD, Brian H. Rowe, MD, MSc, CCFP(EM), Darryl J. Adamko, MD, FRCPC Journal of Allergy and Clinical Immunology Volume 127, Issue 3, Pages 757-764.e6 (March 2011) DOI: 10.1016/j.jaci.2010.12.1077 Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Creating a metabolomic model of asthma in outpatient clinic versus healthy controls. PLS-DA analysis (SIMCA-P 11) of urine metabolite levels for outpatient asthma compared with healthy control children. A, VIP ranking the metabolites according to their significance in the model. B, Scaled and centered metabolite differences between groups shown as the coefficients of variation plot. C, Three-dimensional scatter plot displaying the model’s degree of separation between outpatient asthma (circles) and healthy controls (squares; R2 = 0.72; Q2=0.67). Error bars represent 95% CIs. Journal of Allergy and Clinical Immunology 2011 127, 757-764.e6DOI: (10.1016/j.jaci.2010.12.1077) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Differentiating asthma from healthy subjects. The PLS-DA algorithm separates groups of data on the basis of a score of 0-1; in this case a value closer to 0 indicates no asthma, and above 0.5 indicates the subject has asthma. A, PLS-DA prediction scores for each subject. B, The accuracy of predicting outpatient asthma or healthy control in the form of an ROC. Error bars represent medians and interquartile ranges. Journal of Allergy and Clinical Immunology 2011 127, 757-764.e6DOI: (10.1016/j.jaci.2010.12.1077) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Stable versus unstable asthma. PLS-DA analysis of children with stable outpatient asthma compared with those having an asthma exacerbation. A, Scaled and centered metabolite differences between groups shown in the coefficients of variation plot. B, Three-dimensional representation of separation between outpatient asthma (open circles) and asthma exacerbation (triangles; R2 = 0.84; Q2 = 0.74). Error bars represent 95% CIs. Journal of Allergy and Clinical Immunology 2011 127, 757-764.e6DOI: (10.1016/j.jaci.2010.12.1077) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Stable asthma, unstable asthma, and healthy controls. PLS-DA analysis results for all 3 groups of children. A, Scaled and centered metabolite differences between groups shown in the coefficients of variation plot. B, Three-dimensional separation among healthy controls (squares), outpatient asthma (open circles), and asthma exacerbation (triangles; R2 = 0.74; Q2 = 0.61). Error bars represent 95% CIs. Journal of Allergy and Clinical Immunology 2011 127, 757-764.e6DOI: (10.1016/j.jaci.2010.12.1077) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparing the concentrations of one metabolite through subjects of 2 groups. Journal of Allergy and Clinical Immunology 2011 127, 757-764.e6DOI: (10.1016/j.jaci.2010.12.1077) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions