Phosphorylation of cytosolic phospholipase A2 by IL-3 is associated with increased free arachidonic acid generation and leukotriene C4 release in human.

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Phosphorylation of cytosolic phospholipase A2 by IL-3 is associated with increased free arachidonic acid generation and leukotriene C4 release in human basophils Katsushi Miura, MD, Walter C. Hubbard, PhD, Donald W. MacGlashan, MD, PhD Journal of Allergy and Clinical Immunology Volume 102, Issue 3, Pages 512-520 (September 1998) DOI: 10.1016/S0091-6749(98)70142-3 Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 1 Expression of cPLA2 in human basophils and contaminating cells. Basophils (Ba) (basophil purity: 98% to 99.4%) and contaminating cells (Co) (basophil purity: <1%), which typically contaminate enriched basophil preparations (mainly lymphocytes and monocytes) were lysed at 2 × 107 cells/mL. Lysates containing 2 × 105 cells or human rh-cPLA2 (10 ng) were subjected to Western blot analysis as described in Methods section. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 2 A, Electrophoretic mobility shift of cPLA2 incubated with IL-3 and PMA. Basophils were incubated with IL-3 (10 ng/mL) or PMA (100 ng/mL) for 15 minutes. Reactions were stopped by ice-cold PAG, and samples were microfuged. Cell pellets were resuspended with ice-cold hypotonic lysis buffer. The cPLA2 mobility shift was examined by Western Blot as described in Methods section. Blot shown is representative of 2 separate experiments. B, Time course of electrophoretic mobility shift of cPLA2 by IL-3. Basophils were incubated with IL-3 (10 ng/mL) for times indicated. cPLA2 mobility shift was examined by Western blot, as described above. Blot shown is representative of 2 separate experiments. C, Treatment of whole-cell lysate with potato acid phosphatase before analysis by electrophoresis and Western blotting. Three lanes show cPLA2 from unstimulated cells, cPLA2 from IL-3 stimulated cells, and cPLA2 from stimulated cells treated with acid phosphatase. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 3 Effect of IL-3 on cPLA2 expression. Basophils were incubated with IL-3 (10 ng/mL) for times indicated. Reactions were stopped by ice-cold PAG, and samples were microfuged. Cell pellets were resuspended with ice-cold hypotonic lysis buffer. Expression of cPLA2 was examined by Western blot for expression, as described in Methods section. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 4 Inhibition of IL-3– or PMA-mediated cPLA2 electrophoretic mobility by PKC inhibitors. Basophils were incubated with bis-indolylmaleimide II (Bis II) or Ro-31-8220 (Ro) for 15 minutes and stimulated with IL-3 (1 ng/mL) or PMA (10 ng/mL) for 15 minutes. Reactions were stopped by ice-cold PAG and microfuged. Pellets were resuspended with ice-cold hypotonic lysis buffer. cPLA2 mobility shift was analyzed by Western blot as described in Methods section. Blot shown is representative of 2 separate experiments. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 5 Electrophoretic mobility shift of cPLA2 incubated with 0.1 or 1 μg/mL ionomycin. Basophils were incubated with 0.1 or 1 μg/mL ionomycin or PMA (100 ng/mL) for 15 minutes. Reactions were stopped by ice-cold PAG, and samples were microfuged. Cell pellets were resuspended with ice-cold hypotonic lysis buffer. cPLA2 mobility shift was examined by Western blot as described in Methods section. Blot shown is representative of 2 separate experiments. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 6 Effect of IL-3 on ionomycin-mediated free AA generation and LTC4 release. Basophils were incubated with or without IL-3 (10 ng/ml) for 15 minutes and stimulated with or without ionomycin (0.1 μg/ml) for 30 minutes. Mass of free AA was determined by means of GC/(NICI)MS. LTC4 was assayed with an LTC4-specific RIA as described in Methods section. Values are means ± SEM of 7 experiments. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 7 A, Dose-dependent electrophoretic mobility shift of cPLA2 incubated with IL-3. Basophils were incubated with indicated concentrations of IL-3 for 15 minutes, and cPLA2 mobility shift was examined by Western blot. Blot shown is representative of 2 separate experiments. B, Dose-dependent effect of IL-3 on free AA generation and LTC4 release. Basophils were incubated with indicated concentrations of IL-3 for 15 minutes and stimulated with or without ionomycin (0.1 μg/mL) for 30 minutes. Average ± SEM of net release for 3 experiments is shown. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 8 Electrophoretic mobility shift of cPLA2 incubated with C5a or fMLP. Basophils were incubated with C5a (50 ng/mL) (A) or fMLP (1 μmol/L) (B) for times indicated, and cPLA2 mobility shift was examined by Western blot. Blot shown is representative of 2 experiments. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 9 Inhibition of C5a- or fMLP-mediated cPLA2 electrophoretic mobility by PKC inhibitors. Basophils were incubated with bis-indolylmaleide II (Bis II) or Ro-31-8220 (Ro) for 15 minutes and stimulated with C5a (50 ng/mL) or fMLP (l μmol/L) for 5 minutes. cPLA2 mobility shift was analyzed by Western blot. Blot shown is representative of 2 experiments. Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 10 Effect of C5a on ionomycin-mediated free AA generation and LTC4 release. Basophils were incubated with or without C5a (50 ng/mL) and with or without ionomycin (0.1 μg/mL) for 5 minutes simultaneously. Average ± SEM of net release for 4 experiments is shown. Asterisks indicate statistically significant difference from C5a-mediated free AA or LTC4 releases without treatment of ionomycin (P < .05 as determined by paired t test) Journal of Allergy and Clinical Immunology 1998 102, 512-520DOI: (10.1016/S0091-6749(98)70142-3) Copyright © 1998 Mosby, Inc. Terms and Conditions