Evaluation of multidonor class Ig sequences obtained from H5N1 and H7N9 vaccinated donors. Evaluation of multidonor class Ig sequences obtained from H5N1.

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Evaluation of multidonor class Ig sequences obtained from H5N1 and H7N9 vaccinated donors. Evaluation of multidonor class Ig sequences obtained from H5N1 and H7N9 vaccinated donors. (A) Criteria used to classify paired heavy and light chains from single cells into the three multidonor classes. (B and C) Percentage of H5H1+, H7H3+, H5+H1–, or H7+H3– single cell–sorted cells that expressed a VH1-18 or VH6-1 heavy chain and had an Ig molecular signature that fit one of the three classes indicated in (A). No H5+H1– or H7+H3– B cells expressing VH6-1 were found. The total number of sequences analyzed in each category is indicated above the bar. (D) Percentage of all H5H1+ or H7H3+ cells whose Ig heavy chain sequence matched the molecular signature of one of the three multidonor classes. Statistical significance between the H5H1+ and H7H3+ cells by Fisher’s exact test was P < 0.0001. (E) Each pie chart shows the proportion of H5H1+ (top) or H7H3+ (bottom) cross-reactive B cells in each donor that expressed an Ig heavy/light chain that matched one of the indicated multidonor classes or VH1-69. The volunteer number and total number of sequences obtained from that donor are indicated above each pie chart. Sarah F. Andrews et al. Sci. Immunol. 2017;2:eaan2676 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works