Genomic structure of LTBP-4 around the 3rd 8-Cys repeat.

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Genomic structure of LTBP-4 around the 3rd 8-Cys repeat. Genomic structure of LTBP-4 around the 3rd 8-Cys repeat. (A) PAC/LTBP-4 clone containing genomic sequences from LTBP-4 was used as a template in PCR amplification using LTBP-4.2 and `3rd 8-Cys' primers. The `3rd 8-Cys' primers amplify a region that is absent from LTBP-4Δ8-Cys3rd (for primer sequences see Table 1). The migration of ∼ 4.5 kb and ∼650 bp PCR products in an agarose gel is shown. The molecular weight markers (nt) are shown on the left. (B) Schematic representation of LTBP-4 as well as the exon-intron structure around the 3rd 8-Cys repeat. The sequences obtained from splice-site junctions of introns A, B and C are shown. Katri Koli et al. J Cell Sci 2001;114:2869-2878 © The Company of Biologists Limited 2001