Supplemental Figure 1 Cell proliferation (% of mock)

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Supplemental Figure 1 Cell proliferation (% of mock) 20 40 60 80 100 20 40 60 80 100 120 PC3 DU145 * miR-133a (10nM) miR-1 (10nM) miR-Control (10nM) mock miR-1 (10nM) + miR-133a (10nM) * P<0.0001

Supplemental Figure 4 Non-PCa PCa TAGLN2 LASS2 WDR78 P<0.0001 .000002 .000004 .000006 .000008 TAGLN2 .0002 .0004 .0006 LASS2 WDR78 P<0.0001 P=0.0030 P=0.0030 .000005 .000004 Expression levels of TAGLN2 (Normalized to GAPDH) Expression levels of LASS2 (Normalized to GAPDH) Expression levels of WDR78 (Normalized to GAPDH) .000003 .000002 .000001 0.2 0.4 STXBP4 0.8 1.0 0.6 1.2 0.01 0.02 0.03 0.04 PNP 0.2 0.3 C4orf34 0.5 0.6 0.4 0.7 0.8 P=0.0097 P=0.3749 Expression levels of STXBP4 (Normalized to GAPDH) Expression levels of PNP (Normalized to GAPDH) Expression levels of C4orf34 (Normalized to GAPDH) P<0.0001 Non-PCa PCa

(relative to normal tissue) Supplemental Figure 5 (relative to normal tissue) PNP mRNA expression 2.0 4.0 6.0 8.0 10.0 12.0 PC3 DU145 Non-PCa