Inhibition of residual activity in the TgPKG-HA3’IT mutant by compound 2 augments the motility defect. Inhibition of residual activity in the TgPKG-HA3’IT.

Slides:

Advertisements

Similar presentations

Supplementary Fig. 2 A B ** newly generated ISL1+ motor neurons (%)

Advertisements

Role of Catecholamine Signaling in Brain and Nervous System Functions: New Insights from Mouse Molecular Genetic Study Kazuto Kobayashi Journal of Investigative.

Identification and characterization of the ta2-1 mutant.

by Ines Brandherm, Jennifer Disse, Dagmar Zeuschner, and Volker Gerke

Identification and characterization of a xap5 mutant in Chlamydomonas.

Phosphatase activity associated with Themis is due to its association with Shp1. Phosphatase activity associated with Themis is due to its association.

FL cells are dependent on BCL6 in a NOTCH2-dependent manner.

Two nucleotide-binding sites of ABCE1 act functionally asymmetric.

Fig. 3 TLR8 signaling induces CXCL4 and IFN-α secretion by SSc PDCs.

Fig. 4 CXCL4 potentiates TLR8-mediated activation of SSc pDCs.

Fig. 7 Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors. Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors.

Purification of budding yeast cohesin and its loader.

(A) Growth curves of strains at 30°C in SPP medium or SPP supplemented with 15 μM paclitaxel or 10 μM oryzalin. (A) Growth curves of strains at 30°C in.

Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. GFP,

Multiple distinct molecular mechanisms influence sensitivity and resistance to MDM2 inhibitors in adult acute myelogenous leukemia by Jianting Long, Brian.

Fig. 5 Local gel scaffold for T cell memory response.

Plk1 negatively regulates cortical localization of dynein, NuMA, and LGN during metaphase. Plk1 negatively regulates cortical localization of dynein, NuMA,

The absence of the centrosome changes global microtubule dynamics in a migrating cell. The absence of the centrosome changes global microtubule dynamics.

Volume 14, Issue 1, Pages (January 2008)

Impact of DNMT3A variants on proliferation and differentiation of HSPCs. Impact of DNMT3A variants on proliferation and differentiation of HSPCs. (A) Histograms.

Side-view and apical-view projections of mutant and validation strain biofilms. Side-view and apical-view projections of mutant and validation strain biofilms.

The absence of the centrosome modulates actin filaments organization via FA signaling in a migrating cell. The absence of the centrosome modulates actin.

Modification of the βIII-tubulin C-terminal tail does not affect the incorporation of the protein into the microtubule network, the microtubule architecture,

Fig. 1 LB100 and LB102 specifically inhibit PP2A phosphatase activity and the growth of BCR-ABL+ cells. LB100 and LB102 specifically inhibit PP2A phosphatase.

Plk1 inhibition affects the NuMA turnover at the spindle pole.

Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. Endogenous SMN1 is not recruited to stress granules in HeLa cells.

Reduction or stabilization of TTR levels improves TTR-mediated locomotion impairments. Reduction or stabilization of TTR levels improves TTR-mediated locomotion.

ZFAND5 associates with 26S proteasomes and slows their migration in native PAGE. (A) Purified 26S proteasomes bind directly to ZFAND5. 26S proteasomes.

Binding of recombinant laminin-111, laminin-511, and their EQ mutants to integrins. Binding of recombinant laminin-111, laminin-511, and their EQ mutants.

Recombinant laminins used for in vitro binding assay.

The effects of KY19382 on growth plate senescence and longitudinal bone growth. The effects of KY19382 on growth plate senescence and longitudinal bone.

Numerical analysis of cell morphology of tapetal cells in wild type.

TGF-β stimulates SMAD7 expression via FOXO3.

Ankyrin domain residues permit activation of LRRK2 by Rab29

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Quantification method for the nucleotide occlusion assay.

Fig. 2. Proportion of motile objects and track length

OA and related amines modulate motility and length of schistosomula.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Effect of 14 hit compounds on SMN protein expression in type I SMA patient fibroblast cells (GM03813). Effect of 14 hit compounds on SMN protein expression.

TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics

Pharmacological inhibition of phosphodiesterases can repair phenotypic defects in the TgATPaseP-GC mutant. Pharmacological inhibition of phosphodiesterases.

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

The microtubule-binding region of RECQL4 is required for chromosome alignment. The microtubule-binding region of RECQL4 is required for chromosome alignment.

SYNCRIP mutant generation and initial SYNCRIP mutant LUMIER experiment

Association of NM-HA and NM-GFP with SGs is transient.

FAK is required for LPA-mediated induction of trailing-edge retraction and the restoration of normal morphology. FAK is required for LPA-mediated induction.

SusG LES is required for efficient packing into OMVs

Modification of the β-tubulin C-terminal tail does not affect the partitioning of tubulin between the soluble and polymerized fractions in response to.

Assessing the effect of TNF-α and TRAIL on A2-PPI15–24 CD8 T-cell cytotoxicity. Assessing the effect of TNF-α and TRAIL on A2-PPI15–24 CD8 T-cell cytotoxicity.

Diaphanous regulates the presynaptic actin cytoskeleton.

Diaphanous is required presynaptically for normal synaptic growth.

BCX inhibits lamellipodia formation in lung cells.

A, Mean neurite length (± SEM) per motoneuron in 48 hr cultures after treatment with different concentrations of thrombin (1–1000 nm). A, Mean neurite.

Aggregation and toxicity of α-synuclein in HEK293T cells.

The formation of DSBs after MMS requires MPG activity.

Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. Alkaline Comet assay showing DNA break.

Overexpression of AP2IV-3 enhances tissue cyst formation.

BEZ235 inhibits DNA-PK and enhances G2–M growth arrest after radiation

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

PDL192 and inhibit the growth of xenograft tumors.

BH3-targeted inhibitors drive specific resistance in human cell lines, which can be overcome with alternating or combining inhibitors. BH3-targeted inhibitors.

related to Fig 1. Isolation and characterization of FAPs.

WEE1 inhibition followed by TRAIL treatment results in apoptotic cell death in MB231 cells. WEE1 inhibition followed by TRAIL treatment results in apoptotic.

Fig. 5 Treatment with molecules 13, 14, and 15 decreases HIV-1 R5 infection in human macrophages. Treatment with molecules 13, 14, and 15 decreases HIV-1.

Fig. 6 tlr2 is necessary for SASP activation in vivo.

Presentation transcript:

Inhibition of residual activity in the TgPKG-HA3’IT mutant by compound 2 augments the motility defect. Inhibition of residual activity in the TgPKG-HA3’IT mutant by compound 2 augments the motility defect. Motile fraction and trail lengths of the Pnative-TgPKG-HA3’IT-3′UTRexcised mutant and progenitor (Pnative-TgPKG-HA3’IT-3′UTRfloxed) strains, generated according to Fig S7, were measured by staining with α-TgSag1 antibody after compound two treatment (2 μM, 15 min). A total of 500 parasites for each strain were analyzed to calculate the motile fraction. In total, 100 trails were measured for the progenitor strain, whereas only 30 trails could be evaluated for the mutant because of severe defect (n = 3 assays, mean with SEM). Statistical significance was calculated for each pair of column individually by t test. (*P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001). Özlem Günay-Esiyok et al. LSA 2019;2:e201900402 © 2019 Günay-Esiyok et al.