Reporter gene expression in transgenic zebrafish embryos recapitulates expression of wt1 paralogs. Reporter gene expression in transgenic zebrafish embryos.

Slides:

Advertisements

Similar presentations

Reduced Representation Bisulfite Sequencing to identify targets of Dnmt4 during zebrafish hematopoietic stem cell development Aniket V Gore: Isolation.

Advertisements

Single-cell internalization during zebrafish gastrulation

by Raman Sood, Milton A. English, Christiane L

The Use of Zebrafish to Understand Immunity

Primitive erythropoiesis from mesodermal precursors expressing VE-cadherin, PECAM-1, Tie2, endoglin, and CD34 in the mouse embryo by Masatsugu Ema, Tomomasa.

Volume 10, Issue 9, Pages (May 2000)

Direct fusion lines allow fluorescent visualization of plasmatocyte nuclei, the cytoplasm, or the cytoskeleton in embryos from stage (St) 8 onwards. Direct.

Fig. 6. Effects of CD31-NP targeting in perfused human kidneys.

Muscle Building Developmental Cell

Volume 83, Issue 6, Pages (June 2013)

Expression of bnl in mesodermal bridge‐cells.

Distinct Protein Domains and Expression Patterns Confer Divergent Axon Guidance Functions for Drosophila Robo Receptors Bettina Spitzweck, Marko Brankatschk,

Oceans of opportunity: Exploring vertebrate hematopoiesis in zebrafish

Volume 13, Issue 9, Pages (December 2015)

A Crucial Interaction between Embryonic Red Blood Cell Progenitors and Paraxial Mesoderm Revealed in spadetail Embryos Laurel A. Rohde, Andrew C. Oates,

Single-Cell Transcript Analysis of Pancreas Development

The zebrafish pronephros: A model to study nephron segmentation

Volume 20, Issue 21, Pages (November 2010)

Sonic hedgehog and vascular endothelial growth factor Act Upstream of the Notch Pathway during Arterial Endothelial Differentiation Nathan D. Lawson,

The BMP Signaling Gradient Patterns Dorsoventral Tissues in a Temporally Progressive Manner along the Anteroposterior Axis Jennifer A. Tucker, Keith.

Naohito Takatori, Gaku Kumano, Hidetoshi Saiga, Hiroki Nishida

Volume 17, Issue 6, Pages (November 2016)

Volume 27, Issue 22, Pages e4 (November 2017)

Won-Suk Chung, Didier Y.R. Stainier Developmental Cell

Fig. 2 ALRN-6924 rapidly increases transcription at the p21 locus and affects its bursting dynamics. ALRN-6924 rapidly increases transcription at the p21.

Stefano De Renzis, J. Yu, R. Zinzen, Eric Wieschaus Developmental Cell

Visualisation of metabolic tissues in zebrafish.

Julie E. Cooke, Hilary A. Kemp, Cecilia B. Moens Current Biology

Endothelial Signaling in Kidney Morphogenesis

Autonomous Modes of Behavior in Primordial Germ Cell Migration

Gfi1aa/1b do not mediate Npas4l/Cloche suppression in primitive red blood cells. Gfi1aa/1b do not mediate Npas4l/Cloche suppression in primitive red blood.

Expression of Hox genes, ALK5, GDF11 and ALK4 in Alk5−/− mutants.

Fig. 4. Expression of oxt is undetectable in the med12 mutant but is unaffected by treatment with Wnt inhibitors. Expression of oxt is undetectable in.

Gfi1aa suppresses the endothelial gene expression program in primitive erythroblasts developing from the posterior lateral mesoderm. Gfi1aa suppresses.

Fig. 4. Abdominal aortic aneurysm formation in zebrafish embryos following treatment with Angiotensin II or snuff extract. Abdominal aortic aneurysm formation.

Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle

Single-cell internalization during zebrafish gastrulation

Altered oligodendrocyte progenitor cell (OPC) marker protein expression in two white matter regions of ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos at E15.5. Altered.

Patterns of cell movement in paraxial mesoderm.

hb and pdm1, but not cas, are expressed in the NB7-3 lineage.

Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.

P53 is responsible for the induction of apoptosis, developmental malformations and mortality of G1 and G2tert mutant zebrafish. p53 is responsible for.

The abundance and expression of miRNAs.

Leukocyte infiltration of epidermis and phagocytosis of debris in clint1 mutants. Leukocyte infiltration of epidermis and phagocytosis of debris in clint1.

Fig. 7. Lhx1-RNAi reduces the eye size

BMP signalling is dispensable for early gastruloid patterning.

Comparison ofMyc-induced zebrafish liver tumors with different stages of human HCC and seven mouse HCC models. Comparison ofMyc-induced zebrafish liver.

Misexpression of Tbx4 causes ectopic endodermal budding and tracheo-esophageal fistula. Misexpression of Tbx4 causes ectopic endodermal budding and tracheo-esophageal.

prdm1a is co-expressed with foxd3 and tfap2a at the NPB

EpiDEG efficiently degrades GFP-tagged proteins that localize to different subcellular localizations. epiDEG efficiently degrades GFP-tagged proteins that.

The conserved region upstream of wt1a is required for its expression in the intermediate mesoderm. The conserved region upstream of wt1a is required for.

A 336 bp regulatory region upstream of wt1b is necessary and sufficient for expression in the pronephric glomeruli. A 336 bp regulatory region upstream.

Structure of Wt1 genomic regions in human and zebrafish.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Engineering of D1- or D2-MSN-specific YCnano50 or ArchT-EGFP mice.

Cell-autonomous migration of gata5/smarcd3b-expressing cells to the heart. Cell-autonomous migration of gata5/smarcd3b-expressing cells to the heart. (A-E)

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Alignment of Wt1 genomic regions reveals a highly conserved element upstream of zebrafish wt1a. Alignment of Wt1 genomic regions reveals a highly conserved.

Effects of Chd1-knockdown on global gene expression.

The zebrafish swdp75fm and swdp82mf mutations cause exocrine pancreatic hypoplasia and reduced skin pigmentation. The zebrafish swdp75fm and swdp82mf mutations.

Altered intracellular distributions of acetylated α-tubulin, mitochondria and peroxisomes. Altered intracellular distributions of acetylated α-tubulin,

Fig. 2. Defective development of lymphatic vessels in zebrafish embryos treated with cigarette smoke extract or snuff extract. Defective development of.

Fig. 8. Expression of other genomic-clustered Chrn subunits in the mesodiencephalon.(A) Schematic representation illustrating the assembly of the Chrnb4,

Generation of a genetic model of chronic β-cell inflammation in zebrafish. Generation of a genetic model of chronic β-cell inflammation in zebrafish. (A)

AP2μ2 has an essential function in Wnt/β-Catenin signaling.

Volume 8, Issue 1, Pages (January 2005)

Volume 17, Issue 6, Pages (December 2002)

The neuropod cells. The neuropod cells. (Top left) Neuropod cells synapse with sensory neurons in the small intestine, as shown in a confocal microscopy.

Fgfr1a, fgfr1b, and fgfr2 function redundantly to regulate pectoral fin development. fgfr1a, fgfr1b, and fgfr2 function redundantly to regulate pectoral.

Presentation transcript:

Reporter gene expression in transgenic zebrafish embryos recapitulates expression of wt1 paralogs. Reporter gene expression in transgenic zebrafish embryos recapitulates expression of wt1 paralogs. Transgenic wt1a::GFP (left) and wt1b::GFP (middle) embryos were imaged using a fluorescence microscope. Shown are overlays of dorsal transmission and fluorescence images at the indicated stages. Arrowhead in the bottom left panel marks fusion of the GFP signal at the midline. The schematic representation of wt1a and wt1b expression on the right is based on published data (Bollig et al., 2006; Drummond et al., 1998; Serluca and Fishman, 2001; Wingert et al., 2007) and is confined to expression in the pronephric region; wt1a expression is shown in red, wt1b expression in blue. ep, exocrine pancreas; gl, glomerulus; hpf, hours post fertilization; im, intermediate mesoderm; pt, pronephric tubule. Frank Bollig et al. Development 2009;136:2883-2892 © 2009. © 2009.