Perforin hyperreleasability and depletion in cytotoxic T cells from patients with exacerbated atopic dermatitis and asymptomatic rhinoconjunctivitis allergica

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Perforin hyperreleasability and depletion in cytotoxic T cells from patients with exacerbated atopic dermatitis and asymptomatic rhinoconjunctivitis allergica Andreas Ambach, MD, Bernd Bonnekoh, MD, Harald Gollnick, MD Journal of Allergy and Clinical Immunology Volume 107, Issue 5, Pages 878-886 (May 2001) DOI: 10.1067/mai.2001.114240 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Reduction of perforin-positive lymphocytes in patients with atopy. A, Relative amount of perforin-expressing cells (mean ± SD) among the lymphocytic cell population in 13 patients with exacerbated AD, 9 patients with asymptomatic RCA, and 13 healthy control subjects. B, Corresponding relative portion of perforin-positive cells among CD8hi+ cytotoxic T cells. Journal of Allergy and Clinical Immunology 2001 107, 878-886DOI: (10.1067/mai.2001.114240) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 Representative examples of a dot plot analysis of cells stained with mAbs against CD8 and perforin in an atopic individual (patient with AD) and a healthy volunteer. The lymphocytic population was identified by its characteristics in the forward side scatter and gated. Cells expressing CD8 at high density on their surface (CD8hi+) were defined as CTLs. Note the substantial difference in the CD8hi+ perforin-positive subpopulation between the patient with AD and the healthy control subject. Journal of Allergy and Clinical Immunology 2001 107, 878-886DOI: (10.1067/mai.2001.114240) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Time-dependent upregulation of CD63 on the surface of control lymphocytes after stimulation with ionomycin/PMA. Journal of Allergy and Clinical Immunology 2001 107, 878-886DOI: (10.1067/mai.2001.114240) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 4 Accelerated perforin release from CD8hi+ CTLs of patients with atopy as detected in vitro during ionomycin/PMA stimulation (time course in minutes after onset of stimulation). Representative histogram analyses of CD8hi+ perforin-positive lymphocytes stimulated with ionomycin/PMA are presented for an individual patient with AD and compared with that of a healthy control subject. The y axis indicates counts of CD8hi+lymphocytes. Journal of Allergy and Clinical Immunology 2001 107, 878-886DOI: (10.1067/mai.2001.114240) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 5 Perforin hyperreleasability in CD8hi+ CTLs of patients with atopy. CD8hi+ lymphocytes were analyzed for perforin content over 120 minutes after ionomycin/PMA stimulation. The initial value of the perforin-positive portion of CD8hi+ lymphocytes was set to 100% each. Note that atopic patients released their perforin-containing cytolytic granules twice as fast and in a more complete manner compared with the healthy control subjects (*P < .05, **P < .005). Journal of Allergy and Clinical Immunology 2001 107, 878-886DOI: (10.1067/mai.2001.114240) Copyright © 2001 Mosby, Inc. Terms and Conditions