Fig. 6. F1 trβ mutants accomplish natural metamorphosis.

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Fig. 6. F1 trβ mutants accomplish natural metamorphosis. F1 trβ mutants accomplish natural metamorphosis. (A) Image of F1 offspring produced by mating of the F0 crispants. (B) Mutation sequences from each F1 offspring. PCR products of trβ target site from six F1 froglets were subcloned and sequenced. Sequences highlighted in red and gray denote protospacer adjacent motif (PAM) and protospacer sequence, respectively. Splice donor site is underlined. The deleted and inserted nucleotides are shown by dashes and blue letters, respectively. Capital and small letters indicate exon and intron, respectively. Yuto Sakane et al. Biology Open 2018;7:bio030338 © 2018. Published by The Company of Biologists Ltd