Qianli Zhuang, MD, PhD, Bruce Mazer, MD

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Presentation transcript:

Inhibition of IgE production in vitro by intact and fragmented intravenous immunoglobulin Qianli Zhuang, MD, PhD, Bruce Mazer, MD Journal of Allergy and Clinical Immunology Volume 108, Issue 2, Pages 229-234 (August 2001) DOI: 10.1067/mai.2001.116291 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Effect of intact and fragmented IVIG on IgE production. Human tonsillar B lymphocytes (1 × 106) were cultured with complete medium alone (control) , αCD40 (1 μg/mL), and IL-4 (400 U/mL) in the presence or absence of intact IVIG (10 mg/mL), Fc (4 mg/mL), or F(ab′)2 (6 mg/mL) fragments for 14 days. Supernatants were harvested and subjected to IgE assay by using ELISA. Control cultures with equimolar maltose to IVIG or human serum albumin (0.46 mg/mL) did significantly affect IgE production. Data shown are means ± SEM of 3 independent experiments. **P < .001 versus CD40/IL-4. Journal of Allergy and Clinical Immunology 2001 108, 229-234DOI: (10.1067/mai.2001.116291) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 Dose-dependent inhibition of IgE production by intact and fragmented IVIG. Human tonsillar B lymphocytes (1 × 106) were cultured with αCD40 (1 μg/mL) and IL-4 (400 U/mL) in the presence or absence of intact IVIG, Fc, or F(ab′)2 fragments at concentrations indicated. After 14 days, supernatants were harvested, and IgE was assayed by using ELISA. Data from one of 3 similar experiments is shown. Journal of Allergy and Clinical Immunology 2001 108, 229-234DOI: (10.1067/mai.2001.116291) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Effects of intact and fragmented IVIG on B-lymphocyte proliferation. Cells (2 × 105) were cultured for 96 hours with medium alone (control) , αCD40 (1 μg/mL), and IL-4 (400 U/mL) in the presence or absence of intact IVIG (10 mg/mL), Fc (4 mg/mL), or F(ab′)2 (6 mg/mL) preparations. Cells were pulsed with 1 μCi/well methyl-3H-thymidine for the last 6 hours. 3H-Thymidine incorporation was measured by using liquid scintillation counting. Control cultures with equimolar maltose to IVIG or human serum albumin (0.46 mg/mL) altered proliferation by ±10%, which was not statistically significant. Data shown here are means ± SEM of 5 independent experiments. *P < .05 and **P < .01 versus CD40/IL-4. Journal of Allergy and Clinical Immunology 2001 108, 229-234DOI: (10.1067/mai.2001.116291) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 4 Dose-dependent inhibition of B-lymphocyte proliferation by intact and fragmented IVIG. B lymphocytes (2 × 105) were cultured with αCD40 (1 μg/mL) and IL-4 (400 U/mL) in the absence or presence of intact IVIG, Fc, or F(ab′)2 fragments at concentrations indicated. Cells were pulsed with 1 μCi/well methyl-3H-thymidine for the last 6 hours. 3H-Thymidine incorporation was measured by using liquid scintillation counting. Data shown here are means ± SEM of 3 independent experiments. Journal of Allergy and Clinical Immunology 2001 108, 229-234DOI: (10.1067/mai.2001.116291) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 5 Effects of conventional and aggregated whole molecular IVIG on lymphocyte proliferation. B lymphocytes (2 × 105) were cultured for 96 hours with αCD40 (1 μg/mL) and IL-4 (400 U/mL) in the presence or absence of conventional and aggregated IVIG (see “Methods” section) at indicated concentrations. Cells were pulsed with 1 μCi/well methyl-3H-thymidine for the last 6 hours. 3H-Thymidine incorporation was measured by using liquid scintillation counting. Data shown are means ± SEM of triplicate determinations from one of 2 similar experiments. Journal of Allergy and Clinical Immunology 2001 108, 229-234DOI: (10.1067/mai.2001.116291) Copyright © 2001 Mosby, Inc. Terms and Conditions