Fig. 4. Impaired induction of TH-response genes in trβ crispants

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Fig. 4. Impaired induction of TH-response genes in trβ crispants Fig. 4. Impaired induction of TH-response genes in trβ crispants. mRNA expression of five TH response genes, thyroid hormone receptor β (trβ), krüppel-like factor 9 (klf9), fos-related antigen-2 (fra-2), matrix metallopeptidase 13 (mmp13), and fibroblast activation protein alpha (fapa), was analyzed by qPCR. Impaired induction of TH-response genes in trβ crispants. mRNA expression of five TH response genes, thyroid hormone receptor β (trβ), krüppel-like factor 9 (klf9), fos-related antigen-2 (fra-2), matrix metallopeptidase 13 (mmp13), and fibroblast activation protein alpha (fapa), was analyzed by qPCR. Uninjected control tadpoles and trβ crispants at pre-metamorphic stage (stage 52–54) were treated with or without 10 nM T3 for 3 days and at least four tadpoles were pooled per sample (n=3) in three independent experiments. The expression levels were normalized by rpl8. Expression changes by T3 induction were calculated relative to those of control animals without T3 treatment. Error bars represent ±s.d. (n=9). The three independent experiments exhibited similar trends. Asterisks indicate significant differences between the uninjected control and trβ crispants: *P<0.005; Welch's t-test. Yuto Sakane et al. Biology Open 2018;7:bio030338 © 2018. Published by The Company of Biologists Ltd