Identification of acetylated peptides and proteins by LC-MS/MS.

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Identification of acetylated peptides and proteins by LC-MS/MS. Identification of acetylated peptides and proteins by LC-MS/MS. (A) Overlap of identified acetylated proteins and peptides from WT and OX mycelia. (B) Distribution of acK sites identified per protein. The numbers of proteins with different numbers of acetylation sites (1 to 13) are shown above the bars. (C) Gene Ontology (GO) term (level 2) distribution for the acetylome of WT and OX mycelia. The proteins were classified by GO annotation based on three categories: biological process, cellular component, and molecular function. (D) KEGG pathway-based enrichment analysis of the acetylated proteins. The KEGG pathway database (http://www.genome.jp/kegg/pathway.html) was used to annotate the pathways of the differentially acetylated proteins. Meiling Liang et al. mSystems 2018; doi:10.1128/mSystems.00270-18