Smad3-KO adipose tissues have increased FA uptake and β-oxidation.

Slides:

Advertisements

Similar presentations

Effect of a 6-day icv ghrelin infusion (2.5 nmol/d) on cumulative food intake (A), body weight gain (B), food efficiency (C), and fat mass gain (D) Claudia.

Advertisements

From: MicroRNA let-7 Regulates 3T3-L1 Adipogenesis

Morphology of white adipose tissue and muscle in S1/3 KO mice of different ages Morphology of white adipose tissue and muscle in S1/3 KO mice of different.

Arterioscler Thromb Vasc Biol

Genetic alterations in the PI3K–PTEN–AKT pathway detected during disease progression and their functional impacts. Genetic alterations in the PI3K–PTEN–AKT.

Volume 1, Issue 2, Pages (February 2005)

Volume 3, Issue 1, Pages (July 2002)

Volume 4, Issue 1, Pages (January 2003)

Volume 17, Issue 1, Pages (September 2016)

Hepatic rRNA Transcription Regulates High-Fat-Diet-Induced Obesity

Haploinsufficiency of the ESCRT Component HD-PTP Predisposes to Cancer

Grzegorz Sumara, Olga Sumara, Jason K. Kim, Gerard Karsenty

Volume 17, Issue 5, Pages (May 2013)

Expression characterizations of PARK2.

Microarray analysis of phospho-Twist1–responsive genes.

(A–B) Box plot representing the median relative expression of CD300LG mRNA in skeletal muscle (A) and in SAT (B) by CD300LG genotype. (A–B) Box plot representing.

(red) & endothelium (green) PMCA4 (green) & DAPI (blue)

Expression of NCoR1∆ID in PAX8−/− mice does not reverse repression of T3-positive target genes. qPCR on the indicated pituitary (A) and neuronal (B) genes.

Hepatic rRNA Transcription Regulates High-Fat-Diet-Induced Obesity

Volume 129, Issue 2, Pages (August 2005)

FIP200 controls Bak expression via maintaining microRNA1198-5p expression. FIP200 controls Bak expression via maintaining microRNA1198-5p expression. (A)

Heat Shock Transcription Factor 1 Is a Key Determinant of HCC Development by Regulating Hepatic Steatosis and Metabolic Syndrome Xiongjie Jin, Demetrius.

(A) Genotyping of the Lats2 and Cre alleles in the PyMT-derived cell lines (see primers location in Fig S2A). (A) Genotyping of the Lats2 and Cre alleles.

Volume 17, Issue 8, Pages (November 2016)

MRNA expression levels of the gene encoding CAR in isolated human islets from control donors, cultured for prolonged time (n=2). mRNA expression levels.

Diabetic FXR KO kidney showed increased lipid accumulation.

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Cellularity of epididymal adipose tissue.

BTG2 regulates gluconeogenic gene expression in diabetic mouse models

Gene expression in islets isolated from diabetic mice.

WASH cKO mice display a normal pancreatic development.

Chronic brain insulin infusion reduces liver TG content independent of changes in body weight and food intake. Chronic brain insulin infusion reduces liver.

MTORC1 regulates basal GLUT4 protein expression independent of VWR exercise. mTORC1 regulates basal GLUT4 protein expression independent of VWR exercise.

Effects of canagliflozin on AMPK, lipid synthesis, and fatty acid oxidation in intact cells. Effects of canagliflozin on AMPK, lipid synthesis, and fatty.

Nrf2−/− mice had higher TGF-β1 transcription and FN expression.

GLUT4 LXRE is required for downregulation of adipose GLUT4 mRNA during fasting and diet-induced obesity. GLUT4 LXRE is required for downregulation of adipose.

Abberrent TGF-β activity causes impaired thermoregulation and excess adiposity in Mfap2−/− mice. Abberrent TGF-β activity causes impaired thermoregulation.

Angiotensin-(1-7) prevents the decreased myosin heavy chain levels and increased atrogin-1 and MuRF-1 expressions of muscle under disuse through the Mas.

TXNIP and caspase-1 protein levels are increased in the adipose tissue of ob/ob mice. TXNIP and caspase-1 protein levels are increased in the adipose tissue.

Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic erythrocytes. Elevation of protein levels of O-GlcNAcase in pre-diabetic and diabetic.

Reduced β-cell mass in MCH-KO mice on normal chow and high-fat diets.

MiRNA expression profiling in adipocytes from wild-type (WT) and leptin-deficient ob/ob mice. miRNA expression profiling in adipocytes from wild-type (WT)

Impaired in vitro adipogenic differentiation parallels elevated HDAC9 expression. Impaired in vitro adipogenic differentiation parallels elevated HDAC9.

Capsinoids (CSNs) and mild cold exposure synergistically promote beige adipocyte biogenesis in inguinal WAT. A: Relative mRNA expression levels of Ucp1,

Reduced OXPHOS expression and increased UCP expression.

CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured SGBS cells during adipocyte differentiation. CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured.

SENP2 overexpression increases FAO by upregulating expression of FAO-associated enzymes in muscle. SENP2 overexpression increases FAO by upregulating expression.

Effect of PP on AT morphology and inflammation after 3 days of treatment. Effect of PP on AT morphology and inflammation after 3 days of treatment. A:

MϕRIP140KD mice show browning in vWAT.

Hepatic fuel metabolism in male 5αR1-KO and WT mice

Hepatic PPARγ deficiency impairs the SCFA-induced reduction in hepatic steatosis. Hepatic PPARγ deficiency impairs the SCFA-induced reduction in hepatic.

MϕRIP140KD mice exhibit improved metabolic phenotypes.

Effects of Rosi treatment on ASKO mice.

AKT thr308 phosphorylation related to total AKT2 protein in SOL muscle (m. soleus) (A) and EDL muscle (m. extensor digitorum longus) (D) and AKT ser472.

Energy stores in quadriceps muscles at rest (white bars) and after treadmill exercise (black bars) in WT and HSL KO mice. Energy stores in quadriceps muscles.

Synapse composition is unaltered in SynDIG1-deficient synapses.

A: Gene expression of β-myosin heavy chain (hypertrophic marker) was analyzed on real-time PCR and normalized against 18S expression. n = 8–11 for each.

Lipoprotein lipase is decreased in skeletal muscle of young, lean IR offspring of T2D parents. Lipoprotein lipase is decreased in skeletal muscle of young,

β-Cell–specific deletion of Phb2 renders mice diabetic.

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

TRAF6 enhances I/R-induced oxidative stress.

Fig. 2 TGF-β1 signaling drives fibrosis in Neu1−/− muscle, which is rescued by AAV-mediated gene therapy. TGF-β1 signaling drives fibrosis in Neu1−/− muscle,

TRAF6 exacerbates I/R-induced neuronal death.

Fig. 4 Effects of hematopoietic restoration of TLR9 on adipose tissue inflammation and insulin resistance. Effects of hematopoietic restoration of TLR9.

Bortezomib induces an NRF2 signature and NRF2 protein in tumor cells from leukemic MCL. Gene sets regulated by bortezomib (Supplementary Tables S3 and.

Chronic Treg reduction results in dermal fibrosis.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

Fig. 5 Effects of in vivo blockade of TLR9 on adipose tissue inflammation and insulin resistance in WT mice. Effects of in vivo blockade of TLR9 on adipose.

A: Diet-specific postprandial responses to glucose and NEFA levels.

Presentation transcript:

Smad3-KO adipose tissues have increased FA uptake and β-oxidation. Smad3-KO adipose tissues have increased FA uptake and β-oxidation. A: Relative fold change in mRNA level of the indicated genes in WT and KO adipose tissue as determined by qPCR. Values are normalized to ribosomal protein L27. Normalized values from WT mice were arbitrarily assigned a value of 1. Only genes with twofold or greater change in expression are presented. Values represent mean ± SEM, n= 3/group. Primer sequences are summarized in Supplementary Table 1. B: Immunoblot analysis of indicated proteins in WT and KO adipose tissues. β-Tubulin was used as loading and transfer control. Representative blots from two mice for each genotype are shown. C–F: The rate of [14C]palmitate uptake (C), [14C]palmitate incorporation into triglycerides (D), lipolysis (E), and [14C]palmitate oxidation (F) at the indicated time points of adipocytes freshly isolated from the epididymal fat pads of WT and KO mice. Data are expressed as mean ± SEM, n= 3/group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT controls. Chek Kun Tan et al. Diabetes 2011;60:464-476 ©2011 by American Diabetes Association