Volume 11, Issue 4, Pages (April 2003)

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Volume 11, Issue 4, Pages 435-443 (April 2003) Solution Structure of MT_nc, a Novel Metallothionein from the Antarctic Fish Notothenia coriiceps Clemente Capasso, Vincenzo Carginale, Orlando Crescenzi, Daniela Di Maro, Elio Parisi, Roberta Spadaccini, Piero Andrea Temussi Structure Volume 11, Issue 4, Pages 435-443 (April 2003) DOI: 10.1016/S0969-2126(03)00044-3

Figure 1 NMR Solution Structure of MT_nc (A) Superposition of the backbone heavy atoms (N, Cα, and C′) of residues 31–60 of the 20-best energy-minimized structures (α domain). The termini are indicated by N and C labels. (B) Superposition of the backbone heavy atoms (N, Cα, and C′) of residues 2–28 of the 20-best energy-minimized structures (β domain). The Cd ions are shown as balls. The termini are indicated by N and C labels. The models were generated with MOLMOL [41]. Structure 2003 11, 435-443DOI: (10.1016/S0969-2126(03)00044-3)

Figure 2 Alignments of Fish and Mammal MTs (A) Superposition of the neon representations of models of the α domains of fish (MT_nc; magenta) and mammal (mouse MT-I; green). (B) Atom representation of the α domain of MT_nc; backbone heavy atoms are represented as magenta balls with dimensions corresponding to their van der Waals radii. (C) Sequence alignment of representative fish and mammal MTs. The regions hosting the crucial Cys residue in typical fish or mammal sequences are boxed with magenta and green linings, for fish and mammal sequences, respectively. The models were generated with MOLMOL [41]. Sequence alignment was performed with CLUSTAL X [44]. Structure 2003 11, 435-443DOI: (10.1016/S0969-2126(03)00044-3)

Figure 3 Surface Electrostatic Potentials of Fish and Mammal MTs (A) Comparison of the surface contact plots of the α domains of metallothioneins of fish (MT_nc; panels I and II for two views related by a C2 axis) and mouse (MT-I; panels III and IV for the two corresponding views related by a C2 axis). (B) Comparison of the surface contact plots of β domains of metallothioneins of fish (MT_nc; panels I and II for two views related by a C2 axis) and mouse (MT-I; panels III and IV for the two corresponding views related by a C2 axis). The surfaces are colored according to the electrostatic potential. Calculation of the electrostatic potential and generation of models were performed with MOLMOL [41]. Structure 2003 11, 435-443DOI: (10.1016/S0969-2126(03)00044-3)

Figure 4 Comparison of the Thiol Group Accessibility and Metal Exchange of Fish and Mammal MTs (A) Titration of reactive cysteinyl groups with DTNB. The reaction was carried out as described in Experimental Procedures. (B) Zn2+/Cd2+ exchange in mouse and fish Zn7MT. The exchange reaction was followed as described in Experimental Procedures, by measuring the formation of the Cd2+-thiolate chromophore. Structure 2003 11, 435-443DOI: (10.1016/S0969-2126(03)00044-3)

Figure 5 2D [1H, 113Cd] Long-Range Correlation Spectrum of N. coriiceps Cd-MT The spectrum was collected at 303 K and 400 MHz proton Larmor frequency, with an evolution time for the heteronuclear coupling of 30 ms. The Cd resonances, labeled I–VII in order of increasing chemical shift, are grouped in β and α domains. Structure 2003 11, 435-443DOI: (10.1016/S0969-2126(03)00044-3)